摘要
采用农杆菌介导玉米萌发种子转化方法,将海藻糖合酶基因(TPS)导入玉米自交系昌7-2中。PCR和Southern blot检测结果表明,目的基因已导入转化植株并整合到受体基因组上。田间抗旱性鉴定和生理生化指标结果显示,转基因植株(株系)的抗旱性明显高于对照。说明海藻糖合酶基因具有抗旱功能,利用植物基因工程技术对其进行玉米抗旱育种是切实可行的。
Using Agrobacerium-mediated transformation method of maize germination seed, trehalose synthase gene TPS was transformed to maize inbred Chang 7-2. The putative transgenic plants were verified by PCR amplification and Southern blot hybridization. The results showed that TPS gene had been transformed into maize plants and further integrated into the genome of transgenic plants. The results of drought-resistant investigation in greenhouse confirmed that the drought-resistant transgenic plant was better than non-transformed plants. In brief, developing drought-resistant breeding by gene engineering technique was a practicable way.
出处
《山西农业科学》
2016年第1期1-4,39,共5页
Journal of Shanxi Agricultural Sciences
基金
山西省自然基金项目(2014011030-2)
山西省财政支农项目(015zzcx-23
2014ZYFZ-11)
山西省农业科学院育种工程项目(11yzgc149)
山西省农业科学院育种基础项目(Yyzgc1317)
关键词
玉米
海藻糖合酶基因
抗旱性
遗传转化
maize（Zea mays L.）
trehalose synthase gene（TPS gene）
drought resistance
genetic transformation
