摘要
建立反相离子对高效液相色谱法测定猪肉中呈味核苷酸5’-尿苷酸二钠（5’-UMP）、5’-胞苷酸二钠（5’-CMP）、5’-鸟苷酸二钠（5’-GMP）、5’-肌苷酸二钠（5’-IMP）和5’-腺苷酸二钠（5’-AMP）含量的方法。应用ZORBAX SB-C18色谱柱,以0.01 mol/L KH2PO4溶液（含1.45 mmol/L四丁基硫酸氢铵,p H4.0）-乙腈（97∶3,V/V）为流动相进行洗脱,紫外检测器在254 nm处进行检测,外标法定量。结果表明,5种呈味核苷酸线性关系良好,相关系数均为0.9999,回收率在92.5%106.0%范围内,相对标准偏差在1.75%4.26%之间,5’-UMP、5’-CMP、5’-GMP、5’-IMP和5’-AMP检出限分别为2.0、2.0、2.0、3.0和3.0 mg/kg。该方法操作简便、灵敏度高、重现性好,适用于猪肉中呈味核苷酸含量的测定。
A method was established for the determination of five flavor nucleotides in pork by ion-pair reversedphase high performance liquid chromatography. Separation and quantification was achieved by using a ZORBAX SB-C18 column,and a mobile phase of 0.01 mol/L KH2PO4 solution（contain 1.45 mmol/L tetrabutylammonium hydrogen sulfate, p H4. 0）- acetonitrile（ 97 ∶ 3, V / V） were used. The detection wavelength was 254 nm. The external standard calibration curves were used for quantification. There were good linear correlations for five flavor nucleotides（R2〉0.9999）. Average spike recovery of five flavor nucleotides was 92.5% 106.0%, with relative standard deviations 1.75%4.26%,the limit of detection for 5’-UMP,5’-CMP,5’-GMP,5’-IMP and5’-AMP being 2.0,2.0,2.0,3.0 and 3.0 mg/kg,respectively. This method was simple,sensitive,reproducible and suitable for the determination of flavor nucleotides in pork.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第2期64-67,共4页
Science and Technology of Food Industry
基金
四川省科技支撑计划项目(2011NZ0071)
关键词
反相离子对高效液相色谱法
猪肉
呈味核苷酸
ion-pair reversed-phase high performance liquid chromatography
pork
flavor nucleotides
