摘要
目的研究中耳胆脂瘤中核因子-κβ(NF-κβ)与晚期糖基化终末产物受体(RAGE)的表达,探究上皮炎症反应在胆脂瘤的发病机制中的作用,寻找可能的中耳胆脂瘤干预治疗措施。方法中耳胆脂瘤手术中收集中耳胆脂瘤标本23例及正常外耳道皮肤组织18例,采用免疫组化法检验两种标本中NF-κβ和RAGE表达分布。结果胆脂瘤上皮组织中NF-κβ表达强度显著高于正常外耳道皮肤组织,两者NF-κβ阳性平均光密度分别为0.442±0.060及0.293±0.047(t=8.654,P<0.01);胆脂瘤上皮组织中RAGE表达强度显著高于正常外耳道皮肤组织,两者RAGE阳性平均光密度分别0.175±0.017及0.133±0.013(t=8.516,p<0.01)。胆脂瘤上皮NF-κβ与RAGE表达具有弱相关性r=0.546(P=0.034)。结论 NF-κβ与RAGE在中耳胆脂瘤上皮中表达增加,可能在胆脂瘤的发生和持续发展中起重要作用。调控NF-κβ与RAGE信号传导通路可能是中耳胆脂瘤治疗干预的有效靶点之一。
Objective To study the roles of NF-Kβ and RAGE in the pathogenesis of middle ear cholesteatoma. Methods Samples of middle ear cholesteatoma （n=23） and normal external ear canal skin （n=l 8） were collected during middle ear surgeries. Expression of NF-Kβ and RAGE was examined by immunohistochemistry. Results All epithelial cells in cholesteatoma revealed abundant plasma expression of NF-Kβ and RAGE, significantly higher than that in normal skin （0.442±0.060 vs 0.293±0.047 and 0.175±0.017 vs 0.133±0.013, respectively; p〈0.01）. There was a weak correlation between NF-Kβ and RAGE expression in middle ear cholesteatoma epithelium tissues （r=0.546, p=0.034）. Conclusion NF-kβ and RAGE may be important factors involved in the occurrence and development of middle ear cholesteatom. The signal pathway of RAGE and NF-Kβ may be a new drug target in non-surgical treatment of middle ear cholesteatoma.
出处
《中华耳科学杂志》
CSCD
北大核心
2015年第4期729-733,共5页
Chinese Journal of Otology
基金
泉州市社会发展科技计划项目(2013Z60)
泉州市卫生局资助
