摘要
采用超临界CO_2反向萃取乙酸乙酯紫草素提取液,超临界CO_2流量为15 L/min,压力为28MPa,温度30℃,紫草色素提取率为5.4%,然后应用超临界干燥法制备高溶解度的紫草色素脂质体。采用响应面优化设计法,以脂质体的水溶解度为优化指标,考察了超临界压力、超声波功率、卵磷脂-辛烯基琥珀酸淀粉钠(SPC-OSAS=1:1)和紫草色素的摩尔比对脂质体溶解度的影响。结果表明:在所选取的3个因素中,超临界的压力对脂质体的水溶性的影响最大,超声波功率次之,当超临界的压力为22.0 MPa,超声波的功率为262 W,卵磷脂-辛烯基琥珀酸淀粉钠(SPC-OSAS)与紫草素的摩尔比为2.05,固定化干燥温度35℃,固定化干燥时间2 h,紫草色素乙醇溶液提取物流速15 L/min时,此条件下可得到的紫草素脂质体,具有最大的水溶解度,达到638.54μg/m L,通过该工艺所制备的紫草素复合纳米粒平均粒径为10 nm,粒径均匀分布;结果准确,重现性好,相对于紫草素原料,紫草素脂质体复合纳米颗粒在80℃的耐热稳定性大大提高。通过对脂质体的HPLC分析,乙酰紫草素的含量最高,达到了30.23%。
Supercritical CO2 extraction with ethyl acetate reverse shikonin extract, the flow rate of CO2 was 15 L/min, the pressure was 28 MPa, and temperature was 30 ℃, the shikonin extraction rate was 5.4%, then supercritical drying method high solubility shikonin composite nanoparticles. Response surface optimization design method, water solubility liposomes to optimize the index, the effects of supercritical pressure, ultrasonic power, Lecithin-octenylsuccinate sodium starch（SPC-OSAS=1：1） with shikonin and the molar ratio on the solubility of the liposomes. The results showed that in three selected factors, the influence of the supercritical pressure on the water-soluble liposome maximum, followed by ultrasonic power, when the supercritical pressure was 22.0 MPa, ultrasonic power was 262 W, Lecithin-octenylsuccinate sodium starch（SPC-OSAS） and the molar ratio of 2.05 shikonin, fixed drying temperature was 35 ℃, the immobilization drying time was two hours, shikonin solution flow rate was 15 L/min, the maximum available under this condition solubility was 638.54 μg/m L, shikonin compound prepared by the process of nanoparticles average diameter of 10 nm, uniform particle size. The method was accurate, reliable and reproducible. With respect shikonin materials shikonin liposome complex nanoparticles at 80 ℃. The thermal stability was greatly improved. Liposomes by HPLC analysis, the content of acetyl shikonin the highest, reaching up to 30.23%.
出处
《食品科技》
CAS
北大核心
2016年第1期255-261,共7页
Food Science and Technology
关键词
超临界干燥
紫草色素
脂质体
响应面
supercritical drying
shikonin
liposomes
response surface
