摘要
目的:观察思密达联合乌司他丁(UTI)给药对急性百草枯(PQ)中毒大鼠血浆肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)的干预作用,探讨其可能的作用机制。方法:将104只SD大鼠随机分为生理盐水对照组(A组)8只、染毒对照组(B组)32只、UTI对照组(C组)32只、思密达联合UTI干预组(D组)32只。染毒后第1、3、5、7天分批处死各组存活大鼠,测定血浆TNF-α、TGF-β1含量。结果:B、C、D组血浆TNF-α、TGF-β1水平于染毒后第1天开始明显升高,与A组比较差异均有统计学意义(P<0.01);染毒后第1、3天,C组TNF-α、TGF-β1水平低于B组,差异有统计学意义(P<0.05);染毒后第1、3、5、7天,D组TNF-α、TGF-β1表达明显低于B组,差异均有统计学意义(P<0.01),且D组TNF-α、TGF-β1水平也低于C组,差异有统计学意义(P<0.05,P<0.01)。结论:思密达联合UTI能够有效降低PQ中毒大鼠TNF-α、TGF-β1水平,减轻PQ对大鼠的损伤作用。
Objective：To evaluate the effect of Smecta and utinastatin（UTI）on tumor necrosis factor-a（TNF-α）and transforming growth factor beta-1（TGF-β1）in rats with paraquat（PQ）intoxication,and investigate its mechanism.Method：One hundred and four healthy adult SD rats were randomly divided into group A,group B,group C,group D.Group B,group C and group D were treated intragastrically with PQ at 60mg/kg,Group C given UTI,Group D given UTI and smecta,while only saline was treated the other two groups.Live rats in each group were sacrificed on 1、3、5、7d,for the determination of TNF-α,TGF-β1in plasma of rats.Result：The leves of TNF-α,TGF-β1increased on 1dafter PQ intoxication in group B,group C and group D,and peaked on 7dlater,with statistical difference from group A（P〈0.01）;Compared with group B,the leves of TNF-α,TGF-β1obviously reduced in group C on 1、3d,with statistical difference（P〈0.05）.Compared with group B,the leves of TNF-α,TGF-β1obviously reduced in group D,with statistical difference（P〈0.01）.Compared with group C,the leves of TNF-α,TGF-β1obviously reduced in group D on 1、3、5、7d,with statistical difference（P〈0.05,P〈0.01）.Conclusion：Smecta and UTI reduced PQ plasma the expression of TNF-αand TGF-β1.
出处
《临床急诊杂志》
CAS
2016年第1期53-55,共3页
Journal of Clinical Emergency
