摘要
以多糖提取率为指标,以料液比（g∶m L）、提取温度和提取时间为影响因素进行热水浸提银耳多糖的单因素试验,在此基础上应用Box-Behnken进行响应面分析。得到较佳工艺条件：料液比为1∶60,提取时间为5.57 h,提取温度为97.18℃,理论多糖提取率为20.52%,实际测得值为20.25%,说明该方法可靠。采用MTT（四甲基偶氮唑盐）实验和二氢乙啶（DHE）染色实验检测水提银耳多糖对人皮肤成纤维细胞（HSF）的毒性。MTT实验表明,银耳多糖溶液质量浓度在0~200 mg/L范围内HSF成活率为1,DHE染色实验结果为阴性,说明水提银耳多糖对HSF没有毒性。
Single factor tests for hot water extraction of Tremella fuciformis polysaccharide were conducted with polysaccharide yield as the criteria index as well as solid to solvent ratio（ g∶m L）,extraction time and extraction temperature as the influencing factors. Based upon the test results,a response surface analysis was derived according to Box- Behnken central composite experimental design. The optimal extraction conditions were obtained as following： solid to solvent ratio,1 ∶ 60; extraction time,5. 57 h; extraction temperature,97. 18 ℃. Under the above conditions,the yield of Tremella fuciformis polysaccharide reaches 20. 52% while the yield obtained by quantitative analysis is 20. 25%. Such comparison showed that the test method is reliable. Methyl thiazolyl tetrazolium（ MTT） test method and dihydroethidium（ DHE） staining test method were used for detection of toxicity to fibroblast of human skin（ HSF）. When the mass concentration of the polysaccharide is within range of 0- 200 mg / L,the MTT test showed that the survival rate of HSF achieves 1and the DHE staining test showed negative. Such results proved that the Tremella fuciformis polysaccharide has no cytotoxicity on HSF.
出处
《日用化学工业》
CAS
CSCD
北大核心
2016年第2期101-105,共5页
China Surfactant Detergent & Cosmetics
基金
质检公益性行业科研专项(201310132
201410019)
关键词
化妆品
银耳多糖
响应面法
MTT
二氢乙啶染色
cosmetics
Tremella fuciformis polysaccharide
response surface analysis
MTT
DHE
