摘要
为了分离鉴定南美白对虾在冰温贮藏条件下的腐败菌,并分析其腐败能力的大小。通过冻结实验,确定南美白对虾的冰点,在0℃与冰点之间贮藏南美白对虾,从中分离腐败菌,进行形态学观察和分子生物学鉴定,将分离得到的腐败菌接种至灭菌虾汁中,通过产硫化氢,降解蛋白质,产挥发性盐基氮(TVB-N)的测定衡量腐败能力的大小。结果表明:从南美白对虾中分离得到12株腐败菌,分别是Shewanella hafniensis,Acinetobacter johnsonii,Planoccoccus citreus,Bacillus cereus,Acinetobacter beijerinckii,Enterobacter hormaechei,Arthrobacter bergeri,Bacillus licheniformis,未鉴定出的菌株X1和X10。在冰温贮藏条件下,导致南美白对虾腐败的优势腐败菌依次是Shewanella hafniensis、X1、Acinetobacter johnsonii和Acinetobacter beijerinckii。
In order to isolate and identify penaeus vannamei＇s spoilage bacteria and measure their spoilage ability in ice temperature storage conditions,the freezing point of penaeus vannamei was determined based on freezing experiment and the spoilage bacteria were separated from penaeus vannamei which was stored under the temperature between 0℃ and the freezing point, the isolated microbes were identified by morphological observation and molecular biology methods.The separated spoilage bacteria were inoculated into sterilized shrimp juice, hydrogen sulfide, degradated proteins and volatile base nitrogen （TVB-N）were selected to evaluate the spoilage ability.The results showed that 12 strains of spoilage bacteria were isolated from penaeus vannamei,they were Shewanella hafniensis, Acinetobacter johnsonii, Planoccoccus citreus, Bacillus cereus, Acinetobacter beijerinckii, Enterobacter hormaechei, Arthrobacter bergeri, Bacillus licheniformis, of which X1 and X10 may be a potential new species or genus. In ice temperature storage conditions, dominant spoilage bacteria leading to the corruption of penaeus vannamei were Shewanella hafniensis, X1, Acinetobacter johnsonfi and Acinetobacter beijerinckii.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第5期171-176,共6页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31270050)
天津市高等学校创新团队(TD12-5049)
关键词
南美白对虾
冰温贮藏
腐败菌
分离鉴定
腐败能力
penaeus vannamei
ice temperature storage
dominant spoilage bacteria
isolation and identification
spoilage ability
