摘要
[目的]以前期研究诱导获得的滇杨种质为试验材料,比较分析其苗期叶片形态及光合生理与基因剂量的关系,为滇杨多倍体育种提供理论指导。[方法]利用前期研究所获得的滇杨多倍体当年生苗为材料,测定其叶片大小、叶片厚度、栅栏组织厚度、气孔特性及叶绿素含量,并以滇杨二倍体为对照,分析滇杨不同程度多倍化后的叶片形态及光合生理特性。[结果]表明:嵌合体及四倍体滇杨的叶片长、宽均大于二倍体植株;滇杨嵌合体及四倍体叶片厚度分别为二倍体的1.16、1.41倍,栅栏组织厚度分别为二倍体的1.10、1.23倍,叶片单位视野内气孔数明显少于二倍体,气孔体积则大于二倍体,叶绿素总含量分别为二倍体的127%和141%;滇杨嵌合体及四倍体植株的净光合速率在日变化过程中均高于二倍体;气孔导度、蒸腾速率则与二倍体差别较小;嵌合体、四倍体及二倍体的胞间CO2浓度在日变化过程中变化不明显。[结论]滇杨多倍化后,由于其基因数量的增加,在叶片形态及光合生理特性等方面,表现出了明显区别于其二倍体的剂量效应。
[Objective]The morphological and photo-physioligical difference caused by genome dosage of different ploidies Populus yunnanensis were investigated,in order to provide theoretical guidance for P. yunnanensis polyploid breeding. [Method]Polyploid P. yunnanensis seedlings were obtained which were used as materials to determine the leaf size,leaf thickness,palisade tissue thickness,stomata characteristics and chlorophyll content compared with diploid P. yunnanensis. [Result]The results showed that all the leaf sizes of mosaic and tetraploid were larger than diploid. The leaf thicknesses of mosaic and tetraploid were 1. 16 times and 1. 41 times of that of the diploid,while the palisade tissues of mosaic and tetraploid were 1. 10 times and 1. 23 times thicker than that of the diploid.The stomata number of polyploidized leaf surface in per unit visula field was obviously fewer than that of the diploid,but the volum was larger than that of the diploid. The total chlorophyll contents in mosaic and tetraploid were re-spectively 127% and 141% of that of diploid. The net photosynthesis ratios of mosaic and tetraploid were faster than that of the diploid,while the stomatal conductance and transpiration rate of diploid were little different from the diploid,and the intercellular CO2 concentration in the diurnal variation process did not change significantly in these materials. [Conclusion]The obvious genetic dose-effect was put up after the chromosome number increased. The theoretical basis and practical guidance were offered by the research and the important material foundation were provided for polyploidized P. yunnanensis clone as well.
出处
《林业科学研究》
CSCD
北大核心
2016年第1期103-109,共7页
Forest Research
基金
云南省森林灾害预警与控制重点实验室开放基金(ZK14A102)
云南省教育厅科学研究基金重大专项项目(ZD2015014)
云南省林业厅省级林业科技推广项目([2013]TSYN26)
国家林业局林业公益性行业科研专项项目(201104076)
关键词
滇杨
多倍化
遗传效应
分析
Pinus yunnanensis
polyploidy
genetic effect
analysis
