摘要
在对海洋胶原低聚肽、乌鸡低聚肽、玉米低聚肽、小麦低聚肽、大豆低聚肽的基础理化成分和分子量分布进行分析的基础上,对其ACE抑制作用进行了考察。组成分析显示：5种食源性低聚肽中蛋白质含量很高,均达到了85%以上;分子量大多为1000 u以下（均在85%以上）,主要分布在132~576 u范围内。ACE抑制作用实验结果显示：这5种食源性低聚肽的ACE抑制活性均呈显著的量效关系,半抑制浓度IC_（50）值在0.53-2.76 mg/mL之间,其中玉米低聚肽的ACE抑制作用最强,乌鸡低聚肽的ACE抑制作用最弱。研究明确了食源性低聚肽的ACE抑制作用,为其作为降血压保健食品的开发利用提供了一定的理论支持。
On the basis of compositions and molecular weight distribution of marine collagen oiigopeptides（MCOP）,black-bone silky fowl oiigopeptides（BSFOP）,corn oiigopeptides（COP）,wheat oiigopeptides（WOP） and soy oiigopeptides（SOP）,the angiotensin l-converting enzyme（ACE） inhibitory activity of the five kinds of food-origin oiigopeptides were evaluated.Composition analysis indicated that the foodorigin oiigopeptides had high total protein contents which were all higher than 85%.The major molecular weight of the food-origin oiigopeptides were all less than 1000 Da（higher than 85%）,and small peptides with a molecular weight range of 132~576 u were dominated.Results of ACE inhibitory activity tests indicated that the activity showed a dose-response relationship with the concentration of the food-origin oiigopeptides.The median inhibitory concentrations（IC_（50） values） of the food-origin oiigopeptides were in the range of 0.53~2.76 mg/mL COP had the strongest ACE inhibitory activity and BSFOP had the weakest ACE inhibitory activity.This paper confirmed the ACE inhibitory activities of the five kinds of food-origin oligopeptides and provided certain theoretical support for the development of food-origin oligopeptides as antihypertensive healthy food.
出处
《食品科技》
CAS
北大核心
2016年第2期9-13,共5页
Food Science and Technology
基金
国家十二五科技支撑项目(2012BAD33B04-02)
国家高技术研究发展计划(863计划)项目(2013AA102205-02)
北京市科委科技创新基地培育与发展工程项目(Z121106002812040)
科技北京百名领军人才培养工程项目(Z131110000513026)
关键词
食源性低聚肽
理化成分
ACE抑制作用
food-origin oligopeptides
physical compositions
ACE inhibitory activity
