摘要
对于三联吡啶钌(Ru(bpy)_3^(2+))掺杂的SiO_2纳米粒,以聚电解质聚丙烯酸钠和成膜物质Nafion的混合液对纳米粒进行包覆,制备了聚丙烯酸钠和Nafion混合膜包覆的电化学发光(ECL)纳米粒。结果表明:混合膜包覆的纳米粒,相对于Nafion膜的ECL信号增强了13倍。同时,混合膜表面可交换阳离子显著增加,能够通过离子交换固载大量的Ru(bpy)_3^(2+),纳米粒的ECL信号可进一步增强约3倍。混合膜还具有另外一个显著的优势,即通过混合膜的疏水相互作用可以方便地标记生物大分子,标记抗体仍然具有良好的免疫活性。
Ru（bpy）3^2＋-doped SiO2 nanoparticles were covered with the mixture of polyelectrolyte sodium polyaerylate and membrane-forming substance Nation, and then the electrochemilumines- cence （ECL） nanoparticles were acquired. The results show that ECL intensity of the polyacrylate- Nation mixing membrane covered nanopartieles is enhanced 13 times, compared with the Nafion membrane covered nanoparticles. At the same time, there is an obvious increase of the exchangeable cation on the surface of the mixing membrane, and more Ru（bpy）3^2＋ can be immobilized on the surface of the nanoparticles based on ion exchanging, and the corresponding ECL intensity can be enhanced about 3 times. Another significant advantage of the mixing membrane is that the as-pre- pared nanoparticles can label bio-macromolecule more easily based on the hydrophobic interaction. The results show that the labelled antibody still has good immune activity.
出处
《发光学报》
EI
CAS
CSCD
北大核心
2016年第3期310-314,共5页
Chinese Journal of Luminescence
关键词
信号增强
电化学发光纳米粒
聚丙烯酸钠
Nafion混合膜
生物标记
signal enhancement
electroehemilumineseence nanoparticles
sodium polyacrylate
Nation-mixing membrane
bio-labelling
