摘要
刺梨富含各种营养成分,为提高其综合利用,研究以刺梨果实为原料,利用超声波辅助法在单因素试验基础上结合正交试验,探索乙醇体积分数、液料比、超声温度和时间对刺梨多酚提取率的影响,并对刺梨多酚体外抑菌活性进行分析。结果表明,乙醇提取刺梨多酚最佳提取条件为液料比60∶1,乙醇体积百分数70%,提取温度30℃,超声提取40min,刺梨多酚提取率可达15.930mg/g。体外抑菌试验表明,本实验提取的刺梨多酚对大肠杆菌和金黄色葡萄球菌均具有抑菌活性,其最小抑菌浓度均为20mg/m L,对应的抑菌圈直径分别为6.78和7.39mm。
The extraction technology of polyphenols from fruits of Rosa roxburghii Tratt by ultrasonic assistant and their antibacterial activity were studied to improve its comprehensive utilization of Rosa which has various nutrients. Orthogonal experiment was used based on the Single factor result to study the effect of ethanol volume,solvent to material ration and temperature on extraction yeild of polyphenols. The results showed that the best technological parameters were as follows：solvent and raw material 60∶1,ethanol volume 70%,the extraction temperature 30℃ for 40min. The average extraction efficiency of polyphenols was 15.930 mg/g under the optimum extraction condition. The extracted polyphenols showed the obvious antibacterial efficacy to both Escherichia coli and Staphylococcusaureus. The minimum inhibitory concentration of both was 20 mg/mL,and the corresponding diameter of inhibition zone was 6.78 and 7.39 mm,respectively.
出处
《中国食品添加剂》
CAS
北大核心
2016年第2期57-61,共5页
China Food Additives
基金
国家自然基金(31401319)
山西省高校青年学术带头人支持计划(20151006)
山西省青年科技研究基金(2015021146)
太原科技大学博士启动基金项目(HY201301)
关键词
刺梨
多酚
正交试验
工艺
抑菌活性
Rosa roxburghii Tratt
polyphenols
orthogonal experiment
technology
antibacterial activity
