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山东省规模化猪场四种病毒性疾病的抗体检测 被引量:5

Detection of Immune Antibody for Four Viral Infectious Diseases in Large-scale Pig Farms of Shandong Province
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摘要 为及时了解山东省规模化猪场重大病毒性疾病的抗体水平,试验采用进口ELISA试剂盒,分别对山东省不同地区7个规模化猪场分别送检的共224份血清进行猪瘟病毒、猪蓝耳病病毒、猪伪狂犬病毒及猪圆环病毒的抗体检测。结果显示:7个规模化猪场猪瘟、猪蓝耳病、猪圆环病毒病的抗体阳性率分别在20.00%~87.50%、5.00%~93.10%、38.89%~100.00%之间;猪伪狂犬病野毒感染率为0.00%~88.89%,经SPSS 19.0软件分析知7个不同猪场之间各种疾病的抗体阳性率均存在显著差异性(P〈0.05)。根据以上数据对猪场四种病毒性疾病的抗体水平进行分析,为制定完善的免疫防控方案提供重要的依据。 To understand the immune antibody and infectious antibody level of four kinds of swine viral diseases in Shandong province timely,this test applied imported ELISA kits for detection of antibodies against swine fever virus,porcine reproductive and respiratory syndrome virus,porcine pseudorabies virus,porcine circovirus.Using 224 sera collected from large-scale pig farms from seven different regions of Shandong province(named1~7)with 5kinds of ELISA kits.In this study,the antibody positive rate of 4kinds of swine viral diseases was between 20.00%~87.50%,5.00%~93.10%,38.89%~100.00%,20.00%~86.11%respectively,but the infectious rates of pseudorabies wild virus were 0.00%~88.89%in all detected pigs,the positive antibody rate of various diseases between seven different farms exist significant differences(P〈0.05)according SPSS software analysis.Based on the above data,the antibody level of four swine viral diseases can be analyzed and questions in pig farms were observed.Simultaneously,it provides an important base for formulating prevention and control programs.
出处 《家畜生态学报》 北大核心 2016年第3期65-68,共4页 Journal of Domestic Animal Ecology
基金 国家自然科学基金青年科学基金项目(31201889) 山东省现代农业产业技术体系疫病控制岗位(SDAIT-06-021-07) 山东省科技发展计划(2014GNC111011) 山东省农业科学院青年英才培养计划 山东省农业科学院青年科研基金项目(2014QNM40)
关键词 病毒性疾病 ELISA 抗体检测 swine viral diseases ELISA antibody detection
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