摘要
目的：探讨SP70在卵巢癌患者组织中的表达及其与临床病理特征之间的关系。方法：收集65例卵巢癌组织和30例卵巢良性疾病（15例卵巢良性肿瘤,15例卵巢良性囊肿）组织的石蜡切片,采用免疫组化的方法检测其中SP70的表达情况,并使用半定量评分法判定结果,采用卡方检验或Fisher确切概率法分析SP70在卵巢癌患者组织中的表达及其与临床病理特征之间的关系。应用Spearman等级相关分析研究SP70与CA125在卵巢癌中表达的关系。结果：SP70在卵巢癌组织中的表达显著高于卵巢良性疾病组,差异有显著统计学意义（72.3%vs.3.3%,P﹤0.001）。SP70在低分化卵巢癌组织中的阳性率高于中高分化组（84.4%vs.45.0%,P﹤0.05）。Ⅲ~Ⅳ期卵巢癌患者组织中的阳性率亦高于Ⅰ~Ⅱ期组（94.1%vs.64.6%,P﹤0.05）。SP70表达阳性者易发生淋巴结转移（100.0%vs.64.0%,P﹤0.05）。SP70与CA125的表达无显著相关（r=0.039,P〉0.05）,提示有互补作用。结论：SP70在卵巢癌组织中高表达,特异性高,且与卵巢癌的分化程度、临床分期及淋巴结转移密切相关,可为卵巢癌的诊断及预后评估提供参考。联合检测SP70与CA125可以明显提高卵巢癌诊断的敏感性,对卵巢癌的早发现、早诊断具有重要临床意义。
Objective：To investigate the expression of SP70 in hunman ovarian cancer and its correlations with clinicopathological significance. Methods：A case-control study was conducted. Paraffin sections of tumor tissues from 65 patients with ovarian cancer and 30 patients with benign ovarian disease（15 benign ovarian tumor and 15 benign ovarian cyst） were collected. The expression of SP70 was examined by immunohistochemical staining. The results were scored by semiquantitative analysis. Statistical analysis was evaluated by chisquare test or Fisher exact test. The expression of SP70 in hunman ovarian cancer and their clinicopathological significance was analyzed.Relationship between SP70 and CA125 expression in ovarian cancer was analyzed by using Spearman rank correlation analysis. Results：The positive rate of SP70 in ovarian cancer was higher than that in benign ovarian disease（72.3% vs. 3.3%, P﹤0.05）. The positive rate of SP70 in poor differentiation was also higher than that in well and moderate differentiation cancers（84.4% vs. 45.0%, P﹤0.05）. Meanwhile,the high expression of SP70 in ovarian cancer between Ⅰ~Ⅱ stage and Ⅲ~Ⅳ stage was significantly different（94.1% vs. 64.6%, P﹤0.05）.SP70 expression in patients with lymph node metastasis was very strong and there was significant difference between the former and that without lymph node metastasis（100% vs. 64.0%, P﹤0.05）. The expression of SP70 and CA125 showed a complementary effect,but there was no significant correlation（r=0.039, P 〉 0.05）. Conclusion：High expression of SP70 in ovarian cancer showed significant association with clinical stage,differentiation and lymph node metastasis. Therefore, it would be of some value in the evaluation of the diagnosis and prognosis of ovarian cancer. Joint detection of SP70 and CA125 can obviously improve the diagnostic sensitivity of ovarian cancer, having important clinical significance in early detection and diagnosis of ovarian cancer.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第1期65-68,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81371894
81201359
81101322)
江苏省实验诊断学重点实验室(XK201114)
江苏高校优势学科建设工程基金项目
教育部博导基金(20113234110012)
国家临床重点专科建设项目
关键词
卵巢癌
抗体
单克隆
免疫组化
ovarian cancer
antibodies
monoclonal
immunohistochemistry
