摘要
目的：建立同时测定益心饮口服液中丹参素钠、原儿茶醛、毛蕊异黄酮苷、甘草苷、丹酚酸B和丹酚酸A含量的方法。方法：采用高效液相色谱法。色谱柱为Sepax Sapphire-C18,流动相为乙腈-0.2%甲酸（梯度洗脱）,流速为0.8 ml/min,检测波长为283nm,柱温为25℃,进样量为10μl。结果：丹参素钠、原儿茶醛、毛蕊异黄酮苷、甘草苷、丹酚酸B和丹酚酸A检测质量浓度线性范围为25.00-400.00、2.50-40.00、5.00-80.00、6.25-100.00、18.75-300.00、5.00-80.00μg/ml（r=0.999 9、0.999 9、0.999 9、0.999 9、0.999 8、0.999 8）;精密度、稳定性、重复性试验的RSD〈2.0%;加样回收率分别为101.52%-102.24%、96.25%-99.25%、99.80%-101.80%、96.60%-100.00%、96.51%-99.77%、97.09%-102.00%,RSD分别为0.23%、1.06%、0.75%、1.29%、1.20%、1.91%（n=6）。结论：该方法操作简单、准确性高、重复性好,可用于同时测定益心饮口服液中丹参素钠、原儿茶醛、毛蕊异黄酮苷、甘草苷、丹酚酸B和丹酚酸A的含量。
OBJECTIVE：To establish a method for contents determination of salvianic acid A sodium,protocatechualdehyde,calycosin-7-glycoside,liquiritin,salvianolic acid B and salvianolic acid A. METHODS：HPLC was performed on the column of Sepax Sapphire-C18 with mobile phase of acetonitrile-0.2% formic acid（gradient elution）at a flow rate of 0.8 ml/min,the detection wavelength was 283 nm,the column temperature was 25 ℃,and the injection volume was 10 μl. RESULTS：The linear range was 25.00-400.00 μ g/ml for salvianic acid A sodium（r=0.999 9）,2.50-40.00 μ g/ml for protocatechualdehyde（r=0.999 9）,5.00-80.00 μg/ml for calycosin-7-glycoside（r=0.999 9）,6.25-100.00 μg/ml for liquiritin（r=0.999 9）,18.75-300.00 μg/ml for salvianolic acid B（r=0.999 8）and 5.00-80.00 μg/ml for salvianolic acid A（r=0.999 8）;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 101.52%-102.24%（RSD=0.23%,n=6）,96.25%-99.25%（RSD=1.06%,n=6）,99.80%-101.80%（RSD=0.75%,n=6）,96.60%-100.00%（RSD=1.29%,n=6）,96.51%-99.77%（RSD=1.20%,n=6）and97.09%-102.00%（RSD=1.91%,n=6）,respectively. CONCLUSIONS：The method is simple,accurate and reproducible,and can be used for simultaneous determination of salvianic acid A sodium,protocatechualdehyde,calycosin-7-glycoside,liquiritin,salvianolic acid B and salvianolic acid A in Yixinyin oral solution.
出处
《中国药房》
CAS
北大核心
2016年第9期1272-1274,共3页
China Pharmacy
关键词
高效液相色谱法
益心饮口服液
丹参素钠
原儿茶醛
毛蕊异黄酮苷
甘草苷
丹酚酸B
丹酚酸A
HPLC
Yixinyin oral solution
Salvianic acid A sodium
Protocatechualdehyde
Calycosin-7-glycoside
Liquiritin
Salvianolic acid B
Salvianolic acid A
