摘要
目的通过建立兔膝关节骨性关节炎（OA）模型，探讨白细胞介素（IL）-1β、基质金属蛋白酶（MMP）-9在OA滑膜中的表达及其意义。方法60只新西兰大白兔随机分为6组，A1、A2、A3组为模型组，B1、B2、B3组为对照组。A1、A2、A3组用1．6％木瓜蛋白酶膝关节腔内注射，建立兔膝关节OA模型，分别在2、4、6周后处死，观察大体标本形态学变化及标本滑膜中IL-1β和MMP-9的表达情况。结果（1）实验组4周和8周软骨细胞IL-1β和MMP-9细胞分数明显高于对照组，差异有统计学意义（P〈0．05或P〈0．01），实验组软骨细胞IL-1β和MMP-9细胞分数4周较8周组明显增高，差异有统计学意义（P〈0．05）。（2）酶联免疫吸附试验（ELISA）法检测IL-1β、MMP-9的表达：A1组〉B1组、A2组〉B2组、A3组〉B3组（P〈0．05），模型组中Al组〈A2组、A2组〈A3组（P〈0．05）。（3）Westernblot法检测IL-1β蛋白的相对表达水平：模型组滑膜中的IL-1β的相对表达量均高于对照组，A1组（0．80±0．05）〉B1组（0．40±0．02）、A2组（1．08±0．06）〉B2组（0．51±0．03）、A3组（1．26±0．08）〉B3组（0．61±0．03，P〈0．01），且随实验进展时间的延长而增加。A1组〈A2组，A2组〈A3组（P〈0．05）。结论IL-1β和MMP-9的差异表达与OA退变的发展密切相关。
Objective To investigate the expression and significance of matrix metalloprotein -9 （MMP -9） and interleukin （IL） - 1β in the synovial membrane of osteoarthritis （OA） model. Methods Sixty New Zealand white rabbits were randomly divided into 6 groups： AI, A2, A3 groups as model groups, and Bl, B2, B3 groups as the control groups. In A1, A2 and A3 groups, 1.6% papain was injec- ted into the articular cavity to establish the OA model of the knee joints, and the animals were sacrificed at 2nd, 4th and 6th week. The expression of MMP - 9 and IL - 1β in the specimens was observed. Results （ 1 ） At 4th week and 8th week, the MMP - 9 and IL - 113 expression levels in the model groups were sig- nificantly higher than those in the control groups （ P 〈 0. 05 and P 〈 0.01 ）. At 4th week, the MMP - 9 and IL - 1β levels in the model groups were significantly higher than those at 8th week （P 〈 0. 05 ）. （2） The expression levels of IL - 1β and MMP -9 in group A1 were higher than in group B1, group A2 higher than in group B2, group A3 higher than in group B3 （ P 〈 0. 05 ）, group A1 lower than in group A2 and group A2 lower than in group A3 .（P 〈0. 05）. （3） For Western blotting, the content of IL - 1β in model groups was higher than in control groups ： group A1 （0. 80 ± 0. 05 ） higher than in group B1 （0. 02 ± 0. 40 ）, group A2 （ 1.08 ± 0. 06） higher than in group B2 （0.06 ± 1.08 ）, group A3 （0.03 ± 0. 08 ） high- er than in group B3 （0. 61 ±0. 03, P〈0. 01）, and increased with the time in the model groups： group A1 lower than in group A2, and group A2 lower than in group A3 （P 〈 0. 05 ）. Conclusion The differential expression of IL - 113 and MMP -9 is closely related to the development of OA degeneration.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第3期741-743,共3页
Chinese Journal of Experimental Surgery
