摘要
以新疆乌恰县境内自然分布的6个新疆沙冬青(Ammopiptanthus nanus(M.Pop.)Cheng f.)居群的120份样品为研究对象,采用RAPD分子标记对其遗传多样性水平和种群遗传结构进行分析。结果表明:从33个随机引物中筛选出8个多态性较高且扩增稳定的引物,共扩增出41条DNA条带,多态位点26个,多态性比率(PPB)占63.41%,物种水平遗传多样性相对丰富,居群水平遗传多样性水平较低,各居群间的遗传多样性水平差异不大,Nei's基因多样性(h)指数和Shannon's信息多样性指数(I)分别为0.1169和0.1707,居群间遗传分化系数Gst为0.4171,遗传变异主要存在于居群内,居群之间产生了一定的遗传分化,基因流Nm为0.6989。根据UPGMA聚类图,6个居群可划分为两支,一支是吾合沙鲁居群和2个肖尔布拉克居群,另一支是康苏居群、巴音库鲁乡的托帕尔居群和阿合其居群。新疆沙冬青6个自然居群的遗传距离的分子聚类结果与其地理分布位置密切相关。
The genetic diversity and genetic structure of 120 samples from 6 natural populations of Ammopiptanthus nanus（ M. Pop.） Cheng f. in Wuqia country,Xinjiang were studied by using the random amplified polymorphic DNA（ RAPD） markers. 8 high polymorphism and stable amplification primer were selected from 33 primers,which generated 41 bands in total,and among them 26 bands were polymorphic,the percentage of polymorphic loci（ PPB） was 63. 41%,an relatively abundant genetic variation was detected for species level,but low genetic variation was detected for population level,Nei＇s gene diversity index（ H） and Shannon＇s information diversity index（ I） were 0. 1169 and 0. 1707,coefficient of genetic differentiation among populations（ Gst）was 0. 4171,the genetic variation of A. nanus Cheng f. mainly existed in populations,moderate genetic differentiation was found in different populations,gene flow（ Nm） was 0. 6989. According to the results of UPGMA clustering based on the RAPD results,all populations could be divided into two groups,one group including the Wuheshalu（ WHS） population and 2 Xiaoerbulake（ XB1 and XB2） populations,another group was the Kangsu（ KS） populations,Aheqi（ AHQ） and Tuopaer（ TPR） population of Bayinkuluti country,genetic distance clustering results of A. nanus populations were closely related to their geographical distribution.
出处
《干旱区资源与环境》
CSSCI
CSCD
北大核心
2016年第3期74-79,共6页
Journal of Arid Land Resources and Environment
基金
国家级大学生创新创业训练计划项目(TDGCX201238)资助
关键词
新疆沙冬青
遗传多样性
RAPD分析
Ammopiptanthus nanus（M.Pop.） Cheng f.
genetic diversity
RAPD
