摘要
目的建立固相萃取-超高效液相色谱法同时测定蜜饯中柠檬黄、新红、胭脂红、苋菜红、靛蓝、日落黄、诱惑红、亮蓝、赤藓红9种合成色素。方法样品采用乙醇氨提取,阴离子固相萃取小柱净化,经ZORBAX SB-C_（18）色谱柱分离,甲醇和0.02 mol/L乙酸铵为流动相梯度洗脱,流速0.4 m L/min。采用二极管阵列检测器,采集254 nm（0~8.0 min）、300 nm（8.0~9.5 min）、254 nm（9.5~18.0 min）色谱合成图进行外标法定量,并通过与对照品的光谱比对进行确证。结果 9种合成色素在18 min内可有效分离,在0.4~40μg/m L的线性范围内相关系数均为0.9999,平均回收率为80.1%~98.6%,相对标准偏差为1.8%~5.2%,检测限为1~2 ng。结论该方法前处理简单、检测快速、回收率满意且重复性好,可作为蜜饯中9种合成色素的检测方法。
Objective To establish a method for the simultaneous determination of 9 kinds of synthetic pigments（lemon yellow, new red, neucoccin, amaranth, indigotine, sunset yellow, allura red, brilliant blue FCF, and erythrosin B disodium salt） in preserved fruits by solid phase extraction-ultra performance liquid chromatography（SPE-UPLC）. Methods Samples were extracted using ethanolamine and cleaned up on an anion exchanging solid phase extraction column. ZORBAX SB-C_（18） column was applied with mobile phase consisting of methanol and 0.02 mol/L ammonium acetate by gradient elution with a flow rate of 0.4 m L/min. The 9 kinds of synthetic pigments were detected by photodiode array detector and a composite chromatogram with detection wavelength of 254 nm（0~8.0 min）, 300 nm（8.0~9.5 min）, and 254 nm（9.5~18.0 min） was for quantification by an external standard method. Results Nine kinds of synthetic pigments could be effectively separated within 18 min, and the standard curves had a good linearity over the concentration range of 0.4~40 μg/m L. The correlation coefficients were 0.9999. The average recoveries ranged from 80.1% to 98.6% with relative standard deviations（RSDs） of 1.8%~5.2%. The detection limits for the 9 kinds of synthetic pigments ranged from 1 to 2 ng. Conclusion This method has the advantages of simple pretreatment, fast detection, satisfied with recovery rate and good reproducibility, and is suitable for the determination of the 9 kinds of synthetic pigments in preserved fruits.
出处
《食品安全质量检测学报》
CAS
2016年第2期567-574,共8页
Journal of Food Safety and Quality
基金
陕西省科技统筹创新工程项目(2014FWPT-01)~~
关键词
蜜饯
合成色素
固相萃取
超高效液相色谱法
preserved fruits
synthetic pigments
solid phase extraction
ultra performance liquid chromatography
