摘要
以家蚕丝胶为原料,采用Alcalase碱性蛋白酶水解和超滤技术制备丝胶肽段P4。通过4NQO诱导建立肝组织氧化损伤模型;加入丝胶肽P4,考察其对肝组织羟基自由基清除活性、MDA水平、GSH水平和抗氧化酶系水平的影响,探讨丝胶肽P4对4NQO诱导的肝组织损伤的保护作用。结果表明:丝胶肽段P4能够显著降低MDA水平,显著提高羟基自由基的清除活性,GSH水平,抗氧化酶SOD、CAT水平,说明P4对4NQO诱导的肝组织损伤具有良好的保护作用。这为开发丝胶抗氧化肽功能产品提供了理论依据。
The objectives of this study were to prepare silk sericin peptide（P4） from sericin of silkworm（Bombyx mori） by Alcalase enzymatic hydrolysis and ultrafiltration. The models of liver tissue peroxidation induced by 4NQO was established, by the addition of sericin peptide P4, its influence on hydroxyl free radical scavenging activity, MDA level,GSH level and antioxidase levels in the liver tissue were evaluation, to investigate the protective effect of sericin peptide P4 on liver tissue. The results show that Peptide group P4 could increase the level of hydroxyl free radical scavenging activity, CAT, SOD, GSH and decrease the level of MDA, which indicated that peptide group P4 have protective effect on liver tissue peroxidation induced by 4NQO. The work will be a theoretical basis for development of antioxidative functional food production.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2016年第2期44-49,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
辽宁省食品安全重点实验室开放课题(LNSAKF2013017)
