摘要
为建立酵母β-1,3-葡聚糖高效、快速的提取方法,以细胞壁为原料,在传统碱—酶法基础上,辅助微波加热,采用响应面法优化提取工艺,通过二次回归模型分析得出最佳工艺为:微波功率420 W,加热时间5min,酶添加量2 550U/g。该条件下β-1,3-葡聚糖总糖含量预测值为87.44%,验证值为87.94%。
For finding a rapid and environmental friendly way to extract zymosan（beta-1,3-glucan）,a method was established by microwave-assisted heating at low alkaline concentration,which was using the cell wall as a raw material and based on traditional enzyme alkaline.Extraction process was optimized by using response surface method,the optimum process conditions for quadratic regression model analysis were as followed：microwave power 420 W,heating time 5min;the quantity of enzyme 2 550U/g.Under this condition,the total sugar value could reach 87.44%,and the verificatio n results was 87.94%.
出处
《食品与机械》
CSCD
北大核心
2016年第2期145-148,共4页
Food and Machinery
基金
湛江市科技计划项目资助(编号:2013A05006)
2015年国家级大学生创新创业训练计划项目(编号:201510426027)
关键词
酵母
Β-1
3-葡聚糖
微波辅助
碱—酶法
yeast
beta-1
3-glucan
microwave assisted
alkaline enzyme method
