摘要
以三文鱼骨作为原料,以碳酸钙作为钙源制备出海洋骨胶原低聚肽钙,对其总蛋白含量、螯合率、得率进行测定,结果表明总蛋白含量为62.56%±0.26%,螯合率为51.38%±0.09%,螯合物得率为42.06%±0.10%。然后利用反相高效液相色谱(RP-HPLC)对其进行分离纯化,收集得到8个组分峰,利用Q-TOF质谱仪测定肽序列,共分析出22个肽段,分子量大多在1000 u以下。
Calcium-chelating Marine bone collagen oligopeptides （MBCP-Ca）were prepared by using salmon bone as raw material and calcium carbonate as calcium resource.The total protein content, chelating rate and yield were determined. Results showed that the total protein content, chelating rate and yield were 62.56% ± 0.26%, 51.38% ±0.09% and 42.06% ±0.10%, respectively.Then, MBCP-Ca was separated and purified by reversed phase high performance liquid chromatography（ RP-HPLC）.A total of 8 fractions were collected and subjected to Q-TOF mass spectrometer to identify peptide sequences. A total of 22 peptide sequences were identified, and most molecular weights of the peptides were below 1000 u.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第7期49-51,共3页
Science and Technology of Food Industry
基金
国家十二五科技支撑项目(2012BAD33B04-02)
国家高技术研究发展计划(863计划)项目(2013AA102205-02)
北京市科委农村中心国家现代农业科技城成果惠民科技示范工程项目(Z131100003113010)
科技北京百名领军人才培养工程项目(Z131110000513026)
关键词
海洋骨胶原低聚肽
肽钙螯合物
分离纯化
结构鉴定
Marine bone collagen oligopeptides
calcium- chelating peptides
separation and purification
structuralidentification
