摘要
为了获得与花生高油酸基因相连锁的SSR标记,应用于标记辅助选择。本研究选用低油酸品种花育28号和高油酸品种P76配制杂交组合,构建重组自交系(RIL)群体,采用气相色谱法测定亲本和各株系脂肪酸含量,卡方检验结果为RIL群体3个环境下低油酸株系数和高油酸株系数比值均符合3∶1的分离比例,表明P76的高油酸特性由2对隐性基因控制。利用SSR标记和BSA法,对1151对SSR引物进行筛选扩增,筛选出8对差异引物;以这8对引物对RIL群体中的19份低油酸家系(油酸平均含量44.06%)和24份高油酸家系(油酸平均含量80.75%)进行统计分析,获得了2个与花生油酸含量极显著(0.001水平)相关的标记AH1TC5D06和FI298287,其中,标记AH1TC5D06在RIL群体低油酸株系中的检测符合率为84.21%,在RIL群体高油酸株系中的检测符合率为87.50%;标记FI298287在RIL群体低油酸株系中的检测符合率为84.21%,在RIL群体高油酸株系中的检测符合率为75.00%。进而利用AH1TC5D06和FI298287对RIL群体中所有株系进行扩增,检测出AH1TC5D06和FI298287与控制高油酸特性基因的遗传距离为7.4cM和19.8cM。综合分析标记AH1TC5D06和FI298287可用于花生油酸含量的分子标记辅助选择。
To detect SSR markers linked to genes controlling high oleic content,we established RIL population derived from a cross between Huayu28 and P76,detected the fatty acid content of parents and RIL population by gas chromatography（GC）.Genetic analysis indicated that the high oleic trait of P76 is controlled by two recessive genes.Using bulked segregation analysis（BSA）and peanut SSR molecular markers,there were eight SSR primers enabling to distinguish two bulks.Subsequently,the eight SSR primers were used to amplify 19 low oleic acid（44.06%）and 24 high oleic acid content lines（80.75%）.It was found that 2SSR markers AH1TC5D06 and FI298287 were tightly associated with oleic content.AH1TC5D06 and FI298287were used to genotype the total lines of RIL population.The genetic distance between 2SSR markers and gene controlling oleic content were 7.4cM and 19.8cM,respectively.Our results indicated that AH1TC5D06 and FI298287can be used in marker-assisted selection（MAS）for high oleic acid breeding in peanut.
出处
《花生学报》
北大核心
2016年第1期1-7,共7页
Journal of Peanut Science
基金
山东省农业良种工程
青岛民生计划(14-2-3-34-nsh)
山东省农业科学院重大科技成果培育项目(2015CGPY03)
