摘要
目的:探讨益肺宣肺降浊方对血管性痴呆大鼠海马神经细胞凋亡和关键因子c AMP、PKA表达的影响以及其部分作用机制。方法:80只健康SD大鼠随机分为假手术组、模型组、治疗组和对照组,每组20只。假手术组、模型组予盐水灌胃;治疗组予益肺宣肺降浊方,对照组予吡拉西坦。4组均采用Morris水迷宫实验检测大鼠记忆与空间探索能力,采用放射免疫法检测海马c AMP含量,westernblot法检测海马PKA表达。结果:与模型组比较,假手术组、治疗组及对照组逃避潜伏期缩短、跨越平台次数增多、目标象限停留时间延长(P<0.05);c AMP含量和PKA表达增高(P<0.05);与对照组比较差异无统计学意义(P>0.05)。结论:益肺宣肺降浊方可明显提高血管性痴呆大鼠学习记忆功能和空间探索能力,减少神经细胞凋亡及提高海马c AMP、PKA的表达水平,其作用机制之一可能是通过影响c AMP/PKA信号转导通路关键因子c AMP、PKA的表达来实现。
Objective： To explore the effects of Yifei Xuanfei Jiangzhuo fang（ YFXF） on neuronal apoptosis and the expression of c AMP,PKA essential contributing factor of c AMP-PKA signaling pathway in rats with Vascular Dementiaand its part mechanism.Methods： Eighty SD rats were randomly divided into sham operation group,model group,treatment group and control group with 20 in each group.sham operation group and model group are aised by normal saline,treatment group by YFXF,control group by Piracetam.Four groups was tested with Morris water maze experiment testing memory of rats with space exploration ability,c AMP in different groups were investigated by radioimmunoassay and PKA by Westernblot technique.Results： Compared with model group,control group,the treatment group and control group from the incubation period shorten,cross platform number increase,stay target quadrant for a longer time after treatment（ P〈0.05）; the contents of c AMP and PKA increased.（ P〈0.05）; Compared with group had no significance（ P〈0.05）.Conclusion： YFXF can obviously improve the vascular dementia rats learning and memory function and space exploration ability,reduce the nerve cell apoptosis and raise the level of the expression of hippocampal c AMP and PKA.Its affect on key factors（ c AMP and PKA） of the signal transduction pathway of c AMP / PKA may be one of the mechanisms.
出处
《中华中医药学刊》
CAS
北大核心
2016年第4期778-781,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81160435)
广西自然科学基金项目(2013GXNSFBA019147)
广西壮族自治区中医药民族医药自筹经费科研项目(gxzc1212)
广西中医药大学第一附属医院青年科学基金项目(GZYQJ2012007)
