摘要
目的探讨血管生成抑制蛋白1（vashohibin-1,VASH1）、微血管密度（microvessel density,MVD）在非小细胞肺癌（non-small-cell lung cancer,NSCLC）组织中的表达及VASH1与临床病理特征及MVD的关系。方法采用免疫组织化学方法检测72例NSCLC组织、30例癌旁组织、21例肺良性病变组织蜡块中VASH1表达与MVD水平,同时分析VASH1与NSCLC患者临床病理特征的关系,并探讨其与MVD二者之间的相关性。结果 NSCLC、癌旁组织和肺良性病变组织中VASH1蛋白阳性表达率分别为73.6%（53/72）、26.7%（8/30）、14.3%（3/21）,三者间比较差异有统计学意义（χ^2=33.15,P〈0.01）。VASH1的表达在NSCLC的淋巴结转移、TNM分期和分化程度方面差异有统计学意义（均P〈0.05）,且NSCLC组织中VASH1表达与MVD水平亦呈正相关（r=0.434,P〈0.01）。结论 VASH1在NSCLC组织中表达升高,与NSCLC淋巴结转移、TNM分期、组织分化紧密相关,其在NSCLC的发生、发展过程中起重要作用。
Objective To investigate the expression of vashohibin-1（ VASHl） in non-small-cell lung cancer（NSCLC） and its relation to microvessel density（MVD） and clinicopathologic features. Methods Immunohistochemistry assay was used to detect the expression level of VASHl and MVD in 72 samples of NSCLC tissues,30 samples of pericancerous lung tissue and 21 lung tissue samples of benign disease. Results The positive rates of VASH1 expression were73. 6%（53/72）,26. 7%（8/30） and 14. 3%（3/21） in NSCLC tissue,pericancerous tissue,and tissue of benign lung diseases,respectively（ χ^2= 33. 15,P〈 0. 01）. The expression of VASHl was significantly different in patients with different status of lymph node metastasis,TNM stage and differentiation grade in NSCLC tissue（ P〈0. 05）. VASH1 expression and MVD level in NSCLC tissue were positively correlated（ r = 0. 434,P 〈0. 01）. Conclusion The expression of VASHl is up-regulated in NSCLC,and closely associated with lymph node metastasis,TNM stage,differentiation grade and microvessel density of NSCLC.
出处
《实用肿瘤杂志》
CAS
2016年第2期140-144,共5页
Journal of Practical Oncology
