摘要
目的探讨微小核糖核酸-145(miRNA-145)对人结肠癌细胞系Caco-2细胞迁移、增殖及细胞周期等生物学行为的影响。方法选用Caco-2细胞并进行培养,用脂质体转染法分别将miRNA-145及阴性对照miRNA过表达的质粒转入Caco-2细胞,分为对照组和miRNA-145组进行实验。在倒置荧光显微镜下观察细胞转染效率;采用划痕试验检测miRNA-145对Caco-2细胞迁移的影响;噻唑蓝法检测miRNA-145对Caco-2细胞增殖的影响;流式细胞术检测miRNA-145对Caco-2细胞周期的影响。结果与对照组相比,Caco-2细胞过表达miRNA-145后,细胞迁移速度减慢,生长活力明显受到抑制(P<0.05),G1期细胞百分比增高(P<0.05),而G2期及S期细胞百分比则无明显改变(P>0.05)。结论miRNA-145可抑制Caco-2细胞的迁移和增殖,同时可使Caco-2细胞发生G1期阻滞,减慢细胞周期进程。
Objective To investigate the effect of microRNA-145（ miRNA-145） on proliferation,motility and cell cycle distribution of human colon cancer cell line Caco-2 cells. Methods Caco-2 cells were cultured and then the miRNA-145 and negative control miRNA mimics were transfected into Caco-2 cells by the liposome transfection method. The experiments were consists of two groups： miRNA-145 group and negative control group. Under the fluorescence microscope observe the cell transfection efficiency. Wound healing assay was used to detect the impact of miRNA-145 on Caco-2 cell motility. The change of proliferation of Caco-2 cells was detected by thiazolyl blue method. The flow cytometry was used to examine the changes of miRNA-145 on Caco-2 cell cycle distribution. Results Compared to the negative control group,cellular proliferation and motility were significantly inhibited（ P〈0. 05） after over expressing miRNA-145 in Caco-2 cells,and the proportion of G1-stage cells was increased（ P〈 0. 05） while the proportion of G2-stage and S-stage cells had no obvious change（ P 〉0. 05）. Conclusion miRNA-145 can significantly inhibite Caco-2 cell proliferation and motility,led to the cell cycle arrest at G1-stage and slow down cell cycle progression.
出处
《新乡医学院学报》
CAS
2016年第3期186-189,共4页
Journal of Xinxiang Medical University
基金
郑州市科技局重点科技攻关项目(编号:131PPTGG379-2)
河南省科技创新人才项目(编号:2015-27)
