摘要
以巨紫荆为试验材料,研究其开花过程中花瓣内含物的变化及蔗糖等保鲜剂对花瓣抗氧化性的影响。结果表明:初生代谢物可溶性蛋白质及可溶性糖质量分数均呈先升后降的趋势,且均在盛花期为最大值,可溶性淀粉质量分数在初花期显著增加;次生代谢物类黄酮质量分数呈上升趋势,而花色素苷质量分数在盛花期为最大值。说明在生长发育阶段(花蕾期至盛花期)主要积累初生代谢物用于生长需要,衰老阶段(盛花期至凋花后期)主要积累次生代谢物有利于延缓衰老。不同保鲜剂处理对花瓣生长没有显著差异(p<0.05);两个蔗糖和50mg·L-16-BA处理均增加了SOD活性,但与对照相比不存在显著差异(p<0.05),MDA质量摩尔浓度呈下降趋势,MDA质量摩尔浓度与SOD活性呈负相关性;2%蔗糖能够显著提高CAT活性,与对照相比增加了86.0%,POD活性与CAT活性互为补充。总之,抗氧化酶(SOD、POD、CAT)之间协调作用共同清除活性氧(ROS),延缓衰老;巨紫荆内含物形成的最佳时期是盛花期;保鲜剂2%蔗糖处理巨紫荆切枝,花瓣直径大,花径较长,花瓣内的保护酶活性增强,对延缓巨紫荆花期效果最有效。
We investigated the change of petals composition and different antistaling agents on oxidation resistance of cuttings in Cercis gigantea. The content of soluble protein and sugar increased at first then decreased, and reached maximum at full bloom period. Soluble starch content increased significantly at early flowering period. The content of flavonoids increased gradually in whole flowering period, while the flower pigment content reached peak point at full bloom period. The accumulation of primary metabolites was beneficial to the petals growth, on the other hand the accumulation of secondary metabolites was useful for flower development and could delay the flower senescence. The growth of petals had no significant difference between treatments when the cutting branches were immersed indifferent antistaling agent solutions. The SOD enzyme activity and MDA content showed a negative correlation. The SOD enzyme activity increased under two sugar concentration and 50 mg · L^-16-BA treatments, but there was no significant difference compared to the control. While the content of MDA was declined under these treatments. The CAT activity was singnificantly improved, and increased by 86.0% compared to the control at 2% sucrose treatment. The POD activity increased signifficantiy under 50 mg · L^-1 6-BA treatment. The changes of petals composition were mainly accumulated at full bloom period, and the best antistaling agent was 2% sucrose treatment.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2016年第3期77-81,共5页
Journal of Northeast Forestry University
基金
国家林业局"948"项目(2014-4-61)
"十二五"国家科技支撑计划项目(2012BAD21B05)
江苏省高校优势学科建设工程资助项目(PAPD)
关键词
巨紫荆
花期
花瓣内含物
保鲜剂
抗氧化酶
Cercis gigantean
Flowering phase
Petal inclusion
Antistaling agent
Antioxidant enzymes
