摘要
以金铁锁带叶腋的嫩茎为外植体,开展了基本培养基、腋芽诱导和增殖培养较优培养条件的筛选,并对所获得的生根苗进行了移栽练苗试验。结果表明:MS培养基适合作为金铁锁离体培养的基本培养基;金铁锁腋芽诱导培养的适宜培养基为MS+3.0mg/L 6-BA+0.01mg/L TDZ+0.20mg/L NAA,平均诱导芽数为4.15;在培养基1/2MS+0.30mg/L IBA+0.10mg/L NAA+0.3g/L活性炭中进行生根培养,生根率可达89.6%;在腐殖土∶红土∶珍珠岩=1∶1∶1的基质中练苗,金铁锁组培苗的成活率可达90.5%。
The tender stem with leaf axil of Psammosilenetunicoides was used as explants to select the basic culture medium,the best culture condition of axillary bud induction and multiplication culture.The transplanting and exercising of rooting seedling were also be carried out.The results showed that MS culture medium was suited to use as the basic culture medium to vitroculture of P.tunicoides.The good medium for axillary bud induction of P.tunicoides was MS＋3.0mg/L 6-BA＋0.01mg/L TDZ＋0.20mg/L NAA,the average induction buds was 4.15.In rooting meduinm of1/2MS＋0.30mg/L IBA＋0.10mg/L NAA＋0.3g/L CA,the rooting rate was 89.6%;planting in the matrix of humus∶laterite∶perlite=1∶1∶1,the survival rate of P.tunicoides was 90.5%。
出处
《北方园艺》
CAS
北大核心
2016年第8期81-84,共4页
Northern Horticulture
基金
云南省高校林木遗传改良与繁育重点实验室基金资助项目
关键词
金铁锁
腋芽诱导
组织培养
P
tunicoides
axillary bud induction
tissue culture
