摘要
以香菇66#菌株为试材,采用液体发酵方法,研究了菌丝球数量、pH值、还原糖含量、氨基氮含量、纤维素酶活性、漆酶活性、蛋白酶活性等对香菇菌丝生长主要生理指标的影响。结果表明:当香菇菌株在26℃、160r/min摇床振荡培养至第8天时,菌丝球数量最多。此时,发酵液pH 6.5,还原糖和氨基氮含量分别为47.12mg/100mL和11.87mg/100mL,纤维素酶活性、漆酶活性和蛋白酶活性分别为1.25、0.023、0.18U。
Taking Lentinula edodes 66#as test material,using the method of liquid fermentation,the main physiological indexes（mycelial pellet numbers,pH value,reducing sugar content,amino nitrogen content,cellulase activity,laccase activity,protease activity）affecting the mycelial growth of Lentinula edodes was studied.The results showed that mycelial pellet numbers of Lentinula edodes was the most under the conditions with 26℃,160r/min in the rotary vibrator for 8days.At this time,pH value of fermentation solution was 6.5,reducing sugar content was 47.12mg/100 mL,amino nitrogen content was 11.87mg/100 mL,cellulase activity was 1.25 U,laccase activity was 0.023 U,and protease activity was 0.18 U.
出处
《北方园艺》
CAS
北大核心
2016年第8期142-145,共4页
Northern Horticulture
基金
陕西省"13115"科技创新工程计划资助项目(2008IDGC-04)
关键词
香菇
液体发酵
还原糖
氨基氮
酶活性
Lentinula edodes
liquid fermentation
reducing sugar
amino nitrogen
enzyme activity
