摘要
目的：建立同时测定石淋通片中新西兰牡荆苷2、新西兰牡荆苷1、夏佛塔苷含量的方法。方法：采用高效液相色谱法。色谱柱为Phenomenex C_（18）,流动相为甲醇-0.1%磷酸水溶液（梯度洗脱）,流速为0.8 ml/min,检测波长为334 nm,柱温为25℃,进样量为10μl。结果：新西兰牡荆苷2、新西兰牡荆苷1、夏佛塔苷的检测进样量线性范围分别为0.31~3.10、0.28~2.80、0.39~3.90μg（r=0.999 9）;精密度、稳定性、重复性试验的RSD〈3.0%;加样回收率分别为95.2%~100.4%、96.3%~100.8%、95.7%~100.9%,RSD分别为1.6%、1.4%、1.7%（n=9）。结论：该方法简单、灵敏、准确、可靠,重复性好,可用于石淋通片的质量控制。
OBJECTIVE：To establish a method for simultaneous determination of vicexin 2, vicexin 1 and schaftoside in Shilin- tong tablet. METHODS： HPLC was performed on the column of Phenomenex C18 with mobile phase of methanol -0.1% phosphoric acid （gradient elution）at the flow rate of 0.8 ml/min, the detection wavelength was 334 nm, the column temperature was 25℃, and the injection volume was 10 μl. RESULTS： The linear range was 0.31-3.10 ktg for vicexin 2 （r=0.999 9）, 0.28-2.80 μg for vicexin 1 （r=0.999 9） and 0.39-3.90 pg for schaftoside （r=0.999 9）, respectively;RSDs of precision, stability and reproducibili- ty tests were lower than 3.0% ; recoveries were 95.2% -100.4% （RSD=1.6% , n=9）, 96.3% -100.8% （RSD=1.4% , n=9） and 95.7%-100.9% （RSD= 1.7%, n=9）, respectively. CONCLUSIONS： The method is simple, sensitive , accurate,reliable and repro- ducible, and can be used for the quality control of Shilintong tablet.
出处
《中国药房》
CAS
北大核心
2016年第12期1710-1713,共4页
China Pharmacy
