摘要
全基因组测序技术的问世，为基因组研究带来了极大便利，目前已应用于解决许多微生物方面的问题。本文对微生物全基因组测序技术进行了简要概述，主要从病原微生物的发现和培养诊断、病原体特性的快速鉴定、疫情暴发监测及流行病学溯源、疫苗开发及变异监测、宏基因组学以及单细胞测序6个方面，总结归纳了新一代全基因组测序技术在病原学检测中的作用，并对该研究目前存在的问题进行了回顾总结。全基因组测序具有快速、准确及相对便宜的特点，但是就目前来说，将其应用到临床实验室进行常规病原学检测仍存在一些困难。
Whole-genome sequencing of bacteria has recently emerged as a cost-effective and convenient approach for resolving many microbiological questiona Here, the current status of whole-genome sequencing in clinical microbiology and the main problems that exist were reviewed, focusing on six essential tasks： identifying and culturing the species of an isolate, rapid identification of pathogen properties, monitoring the emergence and spread of an epidemic outbreak, developing vaccine and monitoring its variation, metagenomics and single-cell sequencing The authors predicted that the application of whole- genome sequencing will soon be sufficiently fast, accurate and cheap to be used in routine etiological detection, even though there is still a long way to go.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2016年第4期319-321,共3页
Chinese Journal of Laboratory Medicine
关键词
微生物学技术
基因组学
序列分析
宏基因组学
单细胞分析
Microbiological techniques
Genomics
Sequence analysis
Metagenomics
Single-cell analysis
