摘要
目的研究毛叶疏花蔷薇果提取物的急性经口毒性和遗传毒性。方法急性经口毒性采用最大耐受量法;遗传毒性采用鼠伤寒沙门菌回复突变实验、小鼠骨髓嗜多染红细胞微核实验、小鼠精子畸形实验和单细胞凝胶电泳实验等4项致突变生物学实验进行筛选评价。结果大鼠急性经口毒性>15.0 g/kg,小鼠急性经口毒性>20.0 g/kg;在受试剂量水平下,4项遗传毒性实验结果均为阴性。结论在本实验室中采用SPF级动物,遵照国际公认动物伦理和技术规范进行实验,毛叶疏花蔷薇果提取物为无毒物,且未见明显遗传毒性。
Objective To study the acute oral toxicity and genotoxicity of Rosa laxa Retz. var. moUis Yu et Ku cynarrhodion extracts. Methods The acute oral toxicity in mice and rats （maximal tolerance dose, MTD） were observed for 14 days after adminis- tration. Genetictoxicity was evaluated on Salmonella typhimurium reverse mutation assay （Ames test）, in vivo mammalian erythrocyte micronucleus assay, sperm malformation assay in mice and single cell gel electrophoresis （Comet assay）. Results In acute oral toxici- ty study, the behavior, clinical signs and body changes were normal in all experimental animals. LD50〉 15.0 g/kg（rats） and 20.0 g/kg （mice）. In the test dose range, results of the four genotoxicity tests were negative. Conclusions In accordance with internationally recognized animal ethics and technical specifications for the experiments using SPF animals in our lab, the acute oral toxicity of R. laxa Retz. var. mollis Yu et Ku cynarrhodion extracts is classified as nontoxic, and genotoxicity is not observed under the test condition.
出处
《国际药学研究杂志》
CAS
CSCD
北大核心
2016年第2期314-318,324,共6页
Journal of International Pharmaceutical Research
基金
湖南中医药科研计划资助项目(2013106)
关键词
毛叶疏花蔷薇果
提取物
急性经口毒性
遗传毒性
Rosa laxa Retz.var.mollis Yu et Ku cynarrhodion
extracts
acute oral toxicity
genotoxicity
