摘要
为开发看麦娘内生菌生物防治资源,通过离体拮抗试验、生防试验和分子鉴定等方法,研究了野生日本看麦娘内生真菌KMN-1和KMN-11对小麦赤霉病菌的抑菌活性以及菌株的分类地位。离体生物测定结果表明,2株内生菌对小麦赤霉病菌的菌丝生长有明显拮抗作用,KMN-1和KMN-11菌株发酵液抑制小麦赤霉病菌菌丝生长的EC50值分别为6 572.97 mg·L-1、5 218.34 mg·L-1。进一步采用离体麦穗和田间小区系统研究KMN-1和KMN-11发酵产物对小麦赤霉病的防治效果,结果表明KMN-1和KMN-11对小麦赤霉病均表现一定的防治作用。离体麦穗上,KMN-1发酵产物对小麦赤霉病的保护和治疗作用防治效果分别为78.13%、76.52%;小区生防试验中,2株内生菌代谢产物均可降低小麦赤霉病的发病程度。经形态特征和分子学鉴定,初步确定KMN-1菌株为细极链格孢(Alternaria tenuissima),KMN-11菌株是变红镰孢(Fusarium incarnatum)。本研究表明从镰孢属和链格孢属内生菌中寻找生防菌株的前景广阔,为进一步研究开发有潜力的农用抗生素奠定了基础。
In order to develop the application of endophytic biological control of Alopecurus japonicas Steud.,the antifungal activity and taxonomic status of strain KMN- 1 and KMN- 11 was studied. KMN- 1 and KMN- 11 were significant antifungal activity against Fusarium head blight in vitro antagonism test. By the assay of fermentation from KMN- 1 strain and KMN- 11 strain exhibited fungi activity,the value of EC50 inhibiting mycelia growth against Fusarium graminearum was 6 572. 97 mg·L^-1、5 218. 34 mg·L^-1,respectively. The antibiotic bioactivity of metabolites from strain KMN- 1 and KMN- 11 was studied in vitro and vivo bioassay. The fermentation filtrate of strain KMN- 1had exhibited effect on controlling Fusarium graminearum,the efficacy of protective and curative efficacy were above78. 13% and 76. 52% in wheat spikelet test respectively; In field experiment,spraying the fermentation filtrate of strain KMN- 1 and KMN- 11 can also reduce the epidemic degree. By morphological characteristics and molecular identification,KMN- 1 strain is identified as Alternaria tenuissima and KMN- 11 strain is identified as Fusarium incarnatum. Studies show that the prospects for biocontrol strains which separated from Fusarium spp. and Alternaria are very broad,and also laid the foundation for the further research and development potential of agricultural antibiotics.
出处
《核农学报》
CAS
CSCD
北大核心
2016年第3期460-467,共8页
Journal of Nuclear Agricultural Sciences
基金
四川省科技厅应用基础(2012JY0117)
关键词
小麦赤霉病菌
内生真菌
抑菌活性
鉴定
Fusarium graminearum
endophytic fungi
antifungal activity
identification
