摘要
目的观察中医不同治法对糖皮质激素诱导骨质疏松症大鼠骨抗酒石酸酸性磷酸酶含量的影响,探讨中医防治骨质疏松症的作用机制。方法将120只雌雄各半的大鼠随机分为正常对照组、模型对照组、补肾中药组、健脾中药组、活血化瘀中药组和骨疏康中药组6个组,用地塞米松肌注造模。实验结束后,腹主动脉取血处死大鼠,用酶联免疫法检测各组大鼠血清抗酒石酸酸性磷酸酶的含量。结果与正常组比较,模型组大鼠血清TRACP含量升高极为显著(P<0.01);与模型组比较,各治疗组大鼠血清TRACP含量均明显降低,其中以补肾组和骨疏康组降低最为明显,与其他各组比较具有极显著差异(P<0.01)。结论补肾方法通过降低抗酒石酸酸性磷酸酶含量对骨质疏松症具有一定的防治作用。
Objective To observe the effect of different treatment of traditional Chinese medicine（ TCM ） on serum tartrateresistant acid phosphatase（ TRACP） level,and to explore the mechanism of TCM on prevention and treatment of osteoporosis.Methods One hundred and twenty rats（ male / female = 1∶ 1） were randomly divided into 6 groups,including the blank group,the model group,the kidney-tonifying group,the spleen-invigorating group,the blood circulation-activating group,and the Gushukang group. The model was induced by muscular injection of dexamethasone. At the end of the experiment,the rats were sacrificed and the serum was collected. TRAP level was examined using ELISA method. Results Compared to the blank group,TRACP level in the model group increased significantly（ P〈0. 01）. After treatments,all the groups treated with TCM showed a decreased level of TRACP comparing to that in the model group,especially in the kidney-tonifying group and the Gushukang group（ P〈0. 01）.Conclusion Kidney-tonifying therapy may prevent osteoporosis by decreasing TRACP.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2016年第4期393-395,共3页
Chinese Journal of Osteoporosis
基金
国家自然科学基金青年基金(81001485)
辽宁省教育厅高校杰出青年学者成长计划(LJQ2012084)
关键词
骨质疏松症
抗酒石酸酸性磷酸酶
补肾方法
中医
Osteoporosis
Tartrate-resistant acid phosphatase（TRACP）
Kidney-tonifying therapy
Chinese medicine
