摘要
目的探讨鄂尔多斯地区耐甲氧西林金黄色葡萄球菌（MRSA）分离株杀白细胞毒素（PVL）基因分布特点及药物敏感性。方法收集2012年6月～2014年12月临床分离的SCCmec Ⅱ型和Ⅲ型MRSA菌株66株，应用聚合酶链反应对菌株进行PVL基因型检测，用琼脂稀释法测定PVL阳性菌株对常用抗菌药物的敏感性。结果66例MRsA有6例PVL阳性，阳性率9．09％，其中Ⅱ型4株，Ⅲ型2株；6株PVL阳性菌均对万古霉素、替考拉宁、利奈唑胺和对多种非β内酞胺类抗菌药敏感，对β内酞胺类、大环内酯类和克林霉素高度耐药。结论在鄂尔多斯地区MRSA菌株中PVL阳性菌株较低，4株为CA-MRSA，2株为HA_MRSA，若以PVL阳性作为区分MRsA类型的标志有待进一步研究。
Objective To investigate the distribution of Panton-Valentine leukocidin gene Methicillin-resistant Staphylococcus aureus （MRSA） from Mongolian in Ordos,and the antimicrobial susceptibility of clinical isolates of MRSA strains. Methods A total of 66 MRSA isolates were collected from July 2012 to December 2014 of SCCmec Ⅱ and SCCmec Ⅲ. The polymerase chain reaction （PCR） were used to determine PVL gene. The minimal inhibitory concentrations （MICs） of antibioties for all isolates were determined by agar dilution method. Results Among 66 MRSA strains, the PVL genes were found in 9.09% ,4 were identified as SCCmec Ⅱ and 2 as SCCmec Ⅲ. All PVL positive strains were susceptible to vancomycin,teicoplanin, linezolid and non-β-lactam antibiotics,all were resistant to 13-1actam antibiotics, macrolides and clindamycin. Conclusion The PVL gene positive rate of MRSA isolates was low in Ordos. Among 6 PVL gene positive isolates of MRSA,four belonged to CA-MRSA,two belonged to HA-MRSA. However,a considerable sign of MRSA strains might be undecided by PVL gene.
出处
《现代检验医学杂志》
CAS
2016年第2期80-82,共3页
Journal of Modern Laboratory Medicine
基金
内蒙古自然科学基金(2013MS1116)
关键词
耐甲氧西林金黄色葡萄球菌
杀白细胞毒素
聚合酶链反应
琼脂稀释法
methicillin-resistant Staphylococcus aureus
panton-valentine leukocidin
PCR
minimal inhibitory concentrations
