摘要
背景:益气活血的代表方剂补阳还五汤能够改善微循环,改善组织缺氧;降低纤维蛋白原、抗血小板聚集;降低血脂;扩张血管;抗血栓形成,减少新生斑块的破裂,具有多靶点、多途径抗动脉粥样硬化作用。目的:观察益气活血法的代表方剂补阳还五汤对动脉粥样硬化大鼠模型主动脉Rho激酶、组织因子及基质金属蛋白酶2、基质金属蛋白酶9 m RNA表达的影响,探讨补阳还五汤抗动脉粥样硬化的作用机制。方法:维生素D3加高脂饮食诱导大鼠动脉粥样硬化模型。60只大鼠随机分为正常对照组、模型组、补阳还五汤10 g/kg组、补阳还五汤20 g/kg组、辛伐他汀对照组及补阳还五汤预防组,每组10只。造模成功后干预28 d,检测主动脉Rho激酶、组织因子及基质金属蛋白酶2、基质金属蛋白酶9 m RNA表达及血脂水平。结果与结论:1高脂饮食加维生素D3可诱导大鼠形成动脉粥样硬化模型,模型组有动脉粥样硬化斑块形成;2Rho激酶、组织因子及基质金属蛋白酶2、基质金属蛋白酶9 m RNA表达量及血脂水平:正常对照组、补阳还五汤20 g/kg组、辛伐他汀对照组、补阳还五汤预防组均显著低于模型组,差异有非常显著性意义(P<0.01);补阳还五汤10 g/kg组也显著低于模型组,差异有显著性意义(P<0.05);补阳还五汤预防组、补阳还五汤20 g/kg组、辛伐他汀对照组各组间差异无显著性意义(P>0.05);3结果提示益气活血方剂补阳还五汤具有抗动脉粥样硬化作用,降低Rho激酶、组织因子及基质金属蛋白酶2、基质金属蛋白酶9 m RNA水平可能是其作用机制之一。
BACKGROUND： Representative formulae of Qi-Benefiting and Blood-Activating Chinese medicinal herbs Bu Yang Huan Wu Tang （BYHWT） can improve microcirculation and hypoxia, decrease fibrinogen, resist platelet aggregation, reduce blood lipid, dilate blood vessel, resist thrombosis, and reduce neonatal plaque rupture, and has a multi-target, multi-channel anti-atherosclerotic effect. OBJECTIVE： To observe the influence of representative formulae of Qi-Benefiting and Blood-Activating Chinese medicinal herbs BYHWT on aorta Rho kinase, tissue factor, matrix metal oproteinase-2, matrix metal oproteinase-9 mRNA expression in atherosclerosis models, and to explore the action mechanism of BYHWT on anti-atherosclerosis. METHODS： Vitamin D3 and high fat diet induced atherosclerosis in rats. 60 rats were randomly divided into normal control group, model group, 10 g/kg BYHWT therapy group, 20 g/kg BYHWT therapy group, simvastatin control group and BYHWT prevention group （n=10）. At 28 days after model establishment, the expression level of Rho kinase, tissue factor, matrix metal oproteinase-2, matrix metal oproteinase-9 mRNA expression and blood lipid level were detected. RESULTS AND CONCLUSION： （1） High fat diet and vitamin D3 could induce atherosclerosis in rats. Atherosclerotic plaque formed in the model group. （2） Rho kinase, tissue factor, matrix metal oproteinase-2, matrix metal oproteinase-9 mRNA expression and blood lipid were significantly lower in the normal control group, 20 g/kg BYHWT therapy group, simvastatin control group and BYHWT prevention group than in the model group （P 0.05）. （3） Results indicated that Qi-Benefiting and Blood-Activating BYHWT can resist atherosclerosis, down-regulate the expression of Rho kinase, tissue factor, matrix metal oproteinase-2, and matrix metal oproteinase-9 mRNA expression, which may be one of the mechanisms of anti-atherosclerosis.
出处
《中国组织工程研究》
CAS
北大核心
2016年第18期2703-2710,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(81373532)~~
