摘要
目的检测中山地区胃癌患者血清中爱泼斯坦-巴尔病毒(EB病毒)核抗原1-IgA(NA1-IgA)、EB病毒Zta蛋白IgA(Zta-IgA)、衣壳抗原-IgA(VCA-IgA),探讨EB病毒抗体在胃癌患者血清中的表达情况。方法采用酶联免疫吸附试验(ELISA)对100例胃癌患者、200例健康体检者血清中NA1-IgA、Zta-IgA和VCA-IgA的水平进行测定。结果胃癌患者血清中三种EB病毒抗体总阳性率为21.00%,与健康体检者(7.00%)相比,差异具有统计学意义(P<0.001);EB病毒抗体阳性以低分化腺癌多见,双抗体(Zta-IgA和VCA-IgA)阳性的胃癌共有4例且均为低分化腺癌;在低分化腺癌与中高分化腺癌中,EB病毒抗体阳性率分别为25.00%(15/60)、16.67%(5/30)。结论胃癌患者血清中EB病毒抗体表达量升高,双抗体的阳性表达可能与胃癌的恶性程度相关,为EB病毒与胃癌的相关研究提供了参考依据。
Objective To detect three kinds of serum Epstein--Barr virus （EBV） IgA antibodies （inclu- ding core antigen 1 IgA, Zta IgA and capsid protein antigen IgA） levels of gastric cancer patients in Zhongshan District, so as to explore the expression of EBV antibody in serum of gastric cancer patients. Methods The determination of serum EBV core antigen 1 IgA, Zta IgA and capsid protein antigen IgA levels for was per- formed for 100 gastric cancer patients and 200 by using an ELISA technique. Results Compared with the healthy donors （with positive rate of 7.00%）, the total positive rate of the three kinds of EBV antibodies in gastric cancer patients was 21.00%, which showed statistically significant differences （ P 〈0. 001）. Positive EBV antibody occurred mostly in low differentiated adenocarcinoma, 4 cases carried double positive antibodies （Zta IgA and capsid protein antigen IgA） and all of them were pathologically confirmed as poorly differentiated adenocarcinoma. In patients with poorly differentiated adenocarcinoma and medium/high differentiation adeno- carcinoma, the EBV antibody positive rate was 25.00% （15/60） and 16.67% （5/30）. EBV antibody expressions in gastric cancer patients increased. Double antibody positive lated to the malignant degree of gastric cancer, which provided a reference evidence for tionship between EBV and gastric cancer.
出处
《右江民族医学院学报》
2016年第2期146-149,共4页
Journal of Youjiang Medical University for Nationalities
关键词
胃肿瘤
疱疹病毒4型
人
抗体
血清
gastric carcinoma
herpesvirus 4, human
antibody
serum
