摘要
目的探讨马来酸伊索拉定对阿司匹林所致大鼠胃黏膜损伤的保护作用及其机制。方法 60只健康雄性Wistar大鼠随机分成5组:空白对照组、阿司匹林损伤组、马来酸伊索拉定保护组、硫糖铝保护组、奥美拉唑保护组。各保护组分别用马来酸伊索拉定(10 mg/kg)、硫糖铝(600 mg/kg)及奥美拉唑(10 mg/kg)提前给大鼠灌胃,阿司匹林损伤组用生理盐水(9 mg/ml)灌胃,再用阿司匹林(150 mg/kg)灌胃致急性胃黏膜损伤,测量各组胃黏膜溃疡指数(UI)、黏膜损伤积分(EDS)、胃黏膜血流值、一氧化氮(NO)、氨基己糖、白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)的含量,并在显微镜及电镜下观察胃黏膜组织学改变。结果马来酸伊索拉定保护组各项检测指标显示:UI、EDS明显低于阿司匹林损伤组(P<0.01),高于奥美拉唑保护组(P<0.05),略低于硫糖铝保护组(P>0.05);胃黏膜血流值明显高于阿司匹林损伤组和硫糖铝保护组(P<0.05),略低于奥美拉唑保护组(P>0.05);NO含量明显高于阿司匹林损伤组(P<0.01)和奥美拉唑保护组(P<0.05),略低于硫糖铝保护组(P>0.05);氨基己糖含量明显高于阿司匹林损伤组(P<0.01),低于奥美拉唑保护组(P<0.05),略高于硫糖铝保护组(P>0.05);IL-1、TNF-α含量明显低于阿司匹林损伤组、奥美拉唑保护组及硫糖铝保护组(P<0.01)。结论马来酸伊索拉定可以增加胃黏膜血流、NO及氨基己糖含量,同时抑制炎症性细胞因子IL-1、TNF-α的产生,对阿司匹林所致大鼠急性胃黏膜损伤有明显的预防保护作用。
Objective To investigate the protective effects of Maleate irsogladine on Aspirin-induced gastric mucosal injury in rats, and to explore the potential pathogenesis. Methods Sixty healthy male Wistar rats were randomly divided into 5 groups： control group, Aspirin injuried group, Maleate irsogladine protected group, Sucralfate protected group, and Omeprazole protected group. The 3 protected groups were treated with Maleate irsogladine （10 mg/kg） , Sucralfate （600 mg/kg）, and Omeprazole （10 mg/kg） respectively before gastric mucosal injuried was induced. Then the ulcer index （UI）, epithelial damage scoring （EDS）, gastric mucosal blood flow, the contents of nitric oxide （NO）, hexosamine, interleukin-1 （IL-1） and tumor necrosis factor-α （TNF-α） were detected in each group, the pathological changes on histological sections and ultrastructural sections of gastric mucosa were assessed under microscope or electron microscope. Results The indexes of Maleate irsogladine protected group indicated that the UI and EDS were significandy decreased compared with Aspirin injuried group （P 〈 0.01 ） , increased compared with Omeprazole protected group （P 〈 0.05） , decreased compared with Sucralfate protected group （P 〉 0.05 ）. The gastric mucosal blood flow was significantly increased compared with Aspirin injuried group and Sueralfate protected group （P 〈 0.05 ） , decreased compared with Omeprazole protected group （P 〉 0.05）. The content of NO was significantly increased compared with Aspirin injuried group （P 〈 0.01 ） and Omeprazole protected group （P 〈 0.05 ） , decreased compared with Sucralfate protected group （P 〉 0.05）. The content of hexosamine was significantly increased compared with Aspirin injuried group （P 〈0.01 ） , decreased compared with Omeprazole protected group （P 〈0.05 ） , increased compared with Sucralfate protected group （P 〉 0.05 ）. The contents of IL-1 and TNF-α were significantly decreased compared with Aspirininjuried group, Omeprazole protected group and Sucralfate protected group （P 〈 0. 01 ）. Conclusion Maleate irsoglad- ine can increase gastric mucosal blood flow, the contents of NO, hexosamine and reduce the levels of IL-1 and TNF-α, it has a significant protective effect on rats with acute gastric mucosal injury induced by Aspirin.
出处
《胃肠病学和肝病学杂志》
CAS
2016年第5期542-546,共5页
Chinese Journal of Gastroenterology and Hepatology
