摘要
目的探讨气管切开插管大鼠肺组织病理及肺泡灌洗液肺泡表面活性物质相关蛋白A(SP-A)的变化情况。方法将36只雄性SD大鼠采用随机数字表法分为空白对照组(A组)18只、气管切开插管模型组(B组)18只。于建立模型后24 h、72 h、168 h分别取各组大鼠6只,并取右肺组织行病理切片检查,进行Smith评分评估肺组织损伤情况;取左肺肺泡灌洗液检测SP-A浓度。结果肺组织病理结果显示B组大鼠在建模后24 h、72 h、168 h均有不同程度的水肿、肺泡及间质炎症、出血、肺不张等肺组织损伤,各时间点Smith评分均明显高于A组(P<0.05),并随着时间的延长而增高(P<0.05)。B组大鼠肺泡灌洗液SP-A浓度在各时间点均明显高于A组(P<0.05),且随气管切开插管时间的延长而降低(P<0.05)。结论气管切开插管可导致水肿、肺泡炎症、出血、肺不张等肺组织损伤及肺部固有免疫功能紊乱,SP-A有保护肺组织作用,气管切开插管早期SP-A代偿性增高,随后逐渐下降。
Objective To explore the changes of lung tissue pathology and the bronchoalveolar lavage fluid （BALF） concentrations of surfactant-associated protein A（SP-A） in rats with tracheostomy tube. Methods Thirty-six male SD rats were divided into blank control group（ Group A, n=18） and tracheostomy tube model group（ Group B, n = 18）. Six rats were selected in each group at 24,72 and 168 hours after modeling separately. Then the right lung tissues were taken out for the pathological examination, and the Smith score was used to evaluate the injury of lung tissue. The BALF of left lung was collected for detecting the concentration of SP-A. Results The results of lung tissue pathology showed that the lung tissue injuries including edema, alveolar and interstitial inflammation, alveolar and interstitial hemorrhage, and atelectasis with different degrees existed in the rats of Group B at 24,72 and 168 hours after modeling. The Smith score of Group B was higher than that of Group A at each time point （ P 〈 0.05 ）, and increased with the increase of observation time （ P 〈 0.05 ）. The BALF concentration of SP-A in Group B was significantly higher than that in Group A at each time point （ P 〈 0.05 ）, and decreased with the increase of tracheostomy tube time（P 〈 0.05）. Conclusion Tracheostomy tube might lead to lung tissue injuries including edema, alveolar and interstitial inflammation,alveolar and interstitial hemorrhage, atelectasis ,and also lead to the disorder of pulmonary innate immune function. And SP-A owns a protective function for lung tissue. The level of SP-A has a compensatory increase in the early stage of tracheostomy tube and then gradually decreases.
出处
《广西医学》
CAS
2016年第4期449-451,456,共4页
Guangxi Medical Journal
基金
国家自然科学基金(81460687)
广西壮族自治区卫生厅中医药科技专项(GZPT13-36)
