摘要
目的研究熊果酸(UA)对人上皮性卵巢癌SKOV3细胞增殖和凋亡的影响,并探讨其可能的作用机制。方法将处于对数生长期人上皮性卵巢癌SKOV3细胞随机分为5组:正常对照组、熊果酸(40、20、10 mg·L-1)干预组和顺铂(40 mg·L-1)干预组(阳性对照组)。药物干预48 h后,通过四甲基偶氮唑蓝(MTT)比色法测定细胞增值抑制率;通过流式细胞仪分析细胞周期的改变、观察细胞凋亡并计算细胞凋亡率;通过RT-PCR技术检测细胞Bax mRNA和bcl-2 mRNA表达并计算Bax/bcl-2表达比值,Western blot法检测细胞半胱氨酸蛋白酶-3(caspase-3)蛋白表达并进行半定量分析。结果较正常对照组,熊果酸(40、20 mg·L-1)干预组细胞增殖抑制率显著升高(P<0.01),细胞周期被阻滞于G2/M期,细胞凋亡率显著升高(P<0.01);熊果酸(40、20 mg·L-1)干预组细胞中bcl-2 mRNA表达显著下调、Bax mRNA表达显著上调,Bax/bcl-2表达比值显著升高(P<0.01),caspase-3蛋白表达显著上调(P<0.01)。结论熊果酸具有抑制人上皮性卵巢癌SKOV3细胞增殖并促进其凋亡的作用,其作用机制可能与熊果酸能够有效下调bcl-2 mRNA表达、上调Bax mRNA表达、提高Bax/bcl-2表达比值以及上调caspase-3蛋白表达有关。
Objective To investigate the effects and mechanism of ursolic acid（UA） on the proliferation and apoptosis of epithelial o-varian SKOV3 cancer cell.Methods The Logarithmic epithelial ovarian cancer SKOV3 cells were randomized into five groups：nor-mal control group, UA（40、20、10 mg· L-1 ） groups and Cisplatin 40 mg· L-1 group.48 h later, the cellular growth inhibition rate was calculated by MTT, cell cycle and apoptosis rates were analyzed by flow cytometry, the expression of Bax mRNA and bcl -2 mR-NA were detected by RT -PCR, the level of caspase -3 protein expression was detected by Western blot.Results Compared with normal control group, the cellular growth inhibition rates of UA（40、20 mg· L-1 ） groups were significantly increased（P 〈0.01）; the cell cycle was arrested at G2 /M phase, the apoptosis rate was significantly increased（P 〈0.01）; the expression of bcl -2 mRNA was significantly decreased, while the expression of Bax mRNA was significantly increased, and the ratio of Bax/bcl -2 was significantly increased（P 〈0.01）; the expression of caspase -3 protein was significantly increased （P 〈0.01）.Conclusions UA could effective-ly inhibit epithelial ovarian SKOV3 cancer cell proliferation and promote its apoptosis, which was perhaps related to its effects of downreg-ulating the expression of bcl -2 mRNA, raising the expressions of Bax mRNA and caspase -3, and enhancing the ratio of bcl -2/Bax.
出处
《安徽医药》
CAS
2016年第4期647-650,共4页
Anhui Medical and Pharmaceutical Journal
关键词
熊果酸
上皮性卵巢癌
SKOV3细胞
增殖
凋亡
ursolic acid
epithelial ovarian cancer
SKOV3 cell
proliferation
apoptosis
