摘要
目的比较不同的皮肤及瓶盖消毒方法对凝固酶阴性葡萄球菌(CNS)检出率的影响,为临床改进采集流程及提高血培养检测准确性提供依据。方法选取某院新生儿科2012年6月—2014年8月送检的血培养标本,2012年6月—2013年6月血培养标本为对照组(常规消毒采集流程),2013年8月—2014年8月血培养标本为干预组(改进皮肤及瓶盖消毒方法),比较两组标本CNS检出率。结果对照组543份血培养标本检出病原菌41株,阳性率7.55%;干预组853份血培养标本检出病原菌21株,阳性率2.46%。对照组CNS检出率为4.97%(27株),其中耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)12株,检出率为2.21%;干预组CNS检出率为0.82%(7株),其中MRCNS 6株,检出率为0.70%。两组患者CNS及MRCNS的检出率比较,差异均有统计学意义(χ2分别为24.07、5.92,均P<0.05)。结论改进采集血培养标本时皮肤及瓶盖消毒方法,能降低CNS检出率。
Objective To compare different disinfection methods for skin and bottle caps on the detection rate of coagulase negative staphylococcus（CNS）from blood culture,and provide basis for improving collection process and accuracy of blood culture.Methods Blood specimens from the neonatal department of a hospital between June 2012 and August 2014 were collected,blood specimens cultured between June 2012 and June 2013 were as control group（routine disinfection during blood collection process）,and blood specimens cultured between August 2013 and August 2014 were as intervention group（improved disinfection method for skin and bottle caps）,detection rates of CNS between two groups were compared.Results In control group,41 isolates of pathogens were isolated from543 blood culture specimens,positive rate was 7.55%;in intervention group,21 isolates of pathogens were isolated from853 blood culture specimens,positive rate was 2.46%.The detection rate of CNS in control group was 4.97%（n=27）,12（2.21%）of which were methicillin-resistant coagulase-negative staphylococcus（MRCNS）;the detection rate of CNS in intervention group was 0.82%（n=7）,6（0.70%）of which were MRCNS.Difference in detection rates of CNS and MRCNS between two groups were both statistically significant（χ2=24.07,5.92,both P〈0.05）.Conclusion Improving disinfection methods for skin and caps of blood culture bottles during blood collection can decrease the detection rate of CNS.
出处
《中国感染控制杂志》
CAS
北大核心
2016年第5期324-326,共3页
Chinese Journal of Infection Control
关键词
新生儿
血培养
消毒方法
凝固酶阴性葡萄球菌
neonate
blood culture
disinfection method
coagulase negative staphylococcus
