摘要
目的:研究三七总皂苷对拟缺血损伤的脑微血管内皮细胞维甲酸诱导基因-I(Retinoic Acid-inducible Gene-I,RIG-I)的影响。方法:采用原代培养大鼠脑微血管内皮细胞(Brain Microvascular Endothelial Cell,BMEC),氧糖剥夺法制备拟缺血损伤模型,首先用不同浓度梯度的三七总皂苷进行干预,筛选出三七总皂苷的最佳用药浓度,然后分别采用荧光定量PCR和免疫蛋白印迹法检测各组细胞中RIG-I mRNA及蛋白的表达水平。结果:三七总皂苷在22μg/m L浓度时能够显著提高拟缺血损伤脑微血管内皮细胞的活性,同时能够从基因和蛋白水平下调RIG-I的表达。结论:三七总皂苷对拟缺血损伤脑微血管内皮细胞具有保护作用,提示三七总皂苷在缺血性脑损伤中使RIG-I的表达下调可能是其发挥抗炎作用的内在分子机制之一。
Objective： To study the effect of Panax notoginseng saponins（ PNS） on the expression of RIG-I in mimic ischemic cerebral microvascular endothelial cells（ BMECs）. Methods： Rat BMECs were cultured in oxygen-glucose-deprived（ OGD） conditions to mimic cerebral ischemia in vitro. Firstly,the optimal concentration of PNS was determined by various concentration？ gradients of PNS being administrated on OGD-induced BMECs. Then,the expression levels of RIG-I mRNA and protein in each group cells were detected by Q-PCR and Western blot method. Results： PNS can significantly improve the cells activity at 22μg / m L concentration,and decrease the expression of RIG-I in gene and protein levels in the OGD-induced BMECs. Conclusion： PNS has a protective effect on mimic ischemic BMECs,the mechanism may be related to the down-regulation of RIG-I expression,which may be one of the mechanisms of anti-inflammatory effects of PNS in cerebral ischemic injury.
出处
《世界中医药》
CAS
2016年第4期699-702,共4页
World Chinese Medicine
基金
国家自然科学基金项目(编号:81273885)
北京中医药大学研究生自主课题项目(编号:2015-JYB-XS043)
北京市自然科学基金项目(编号:7144223)
关键词
三七总皂苷
脑微血管内皮细胞
维甲酸诱导基因-I
Panax notoginseseng saponins
Cerebral microvascular endothelial cells
Retinoic acid-inducible gene-I
