摘要
利用同源克隆技术,从落叶松-杨栅锈菌(Melampsora larici-populina Kleb.)菌株Wh03中克隆得到酿酒酵母MKK1和MKK2的同源基因,命名为Mlp MKK1/2-WH。测序结果显示,Mlp MKK1/2-WH基因的开放阅读框长度为1 218 bp,编码405个氨基酸。Mlp MKK1/2-WH蛋白具有保守的PKc_Pek1_like和Pkinase结构域。系统进化树分析表明,Mlp MKK1/2-WH与柄锈菌属3种锈菌中的同源蛋白亲缘关系最近。
By using homology-based cloning method,the gene MlpM KK1 / 2- WH was cloned and sequenced from Melampsora larici-populina（ Mlp） Chinese strain Wh03. The full-length ORF of MlpM KK1 / 2-WH was 1 218 bp,encoding 405 amino acid residues. The deduced MlpM KK1 / 2-WH protein contains conserved PKc_Pek1_like and Pkinase domains. By phylogenetic analysis,MlpM KK1 / 2-WH had the closest relationship with the othologs from three rust fungi belonging to the genus Puccinia.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2016年第5期84-87,共4页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(31300544)
西北农林科技大学基本科研业务费(QN2013014
2452015332)
关键词
落叶松-杨栅锈菌
MKK基因
同源克隆
生物信息
Melampsora larici-populina
MKK gene
Homology cloning
Bioinformatical analysis
