摘要
目的:研究宫颈阴道分泌物及胎盘组织中纤维连接蛋白的含量及其与早产相关分子表达量的相关性。方法:选择在我院妇产科住院分娩的早产孕妇60例和足月分娩孕妇60例,分别纳入早产组和对照组,采集宫颈阴道分泌物并测定fFN含量,采集胎盘并测定fFN、SOCS、TLR4、NF-kB含量,采集血清并测定MMP2、MMP3、MMP9、IL-2、IL-6、TNF-α含量,通过超声测定宫颈长度。结果:早产组阴道分泌物中fFN的含量明显增加、而胎盘中fFN的含量无明显变化;宫颈阴道分泌物中fFN阳性患者的宫颈长度以及血清MMP2、MMP3、MMP9、IL-2、IL-6、TNF-α含量明显高于fFN阴性患者,胎盘中TLR4、NF-kB的含量明显高于宫颈阴道分泌物中fFN阴性的患者,SOCS3的含量明显低于宫颈阴道分泌物中fFN阴性的患者。结论:宫颈阴道分泌物中fFN含量增加能够通过TLR4/NF-kB通路、SOCS3通路来增加基质金属蛋白酶分子和炎性因子的表达,进而造成早产的发生。
Objective:To study fibronectin content in cervical ripening and placenta tissue and its correlation with preterm-related molecule expression.Methods:A total of 60 women with preterm delivery and 60 women with term delivery who delivered in Obstetrics Department of our hospital were selected and included in preterm group and control group respectively,cervical ripening was collected to detect fFN content,placenta was collected to detect fFN,SOCS,TLR4 and NF-kB content,serum was collected to detect MMP2,MMP3,MMP9,IL-2,IL-6 and TNF-αcontent,and cervical length was measured by ultrasound.Results:fFN content significantly increased in cervical ripening of preterm group while fFN content in placenta showed no significant changes;cervical length as well as serum MMP2,MMP3,MMP9,IL-2,IL-6 and TNF-αcontent in cervical ripening of fFN-positive patients were significantly higher than those of fFN-negative patients,TLR4 and NF-kB content in placenta were significantly higher than those in cervical ripening of fFN-negative patients,and SOCS3 content was significantly lower than that in cervical ripening of fFN-negative patients.Conclusion:Increase of fFN content in cervical ripening can increase the expression of matrix metalloproteinases and inflammatory factors through TLR4/NF-kB pathway and SOCS3 pathway,thereby leading to premature delivery.
出处
《海南医学院学报》
CAS
2016年第14期1560-1563,共4页
Journal of Hainan Medical University
基金
河源市社会发展计划项目(2013-091)~~
关键词
早产
胎儿纤维连接蛋白
炎性因子
基质金属蛋白酶
Premature delivery
Fetal fibronectin
Inflammatory factors
Matrix metalloproteinases
