摘要
目的观察参芎葡萄糖注射液对兔颈动脉球囊损伤后血管内膜增生的影响。方法选取4月龄健康雄性新西兰兔35只,随机分为空白组、手术组、辛伐他汀组、参芎葡萄糖组、参芎葡萄糖+辛伐他汀组,每组7只。饲养2周后测定血清胆固醇水平,苏木精-伊红染色法(HE)观察血管内膜增生情况,免疫组织化学染色分析血管内膜中膜增殖细胞核抗原(PCNA)、基质金属蛋白酶-9(MMP-9)表达,Western blot法分析颈动脉葡萄糖调节蛋白78(GRP78)表达。结果与空白组相比,手术组血清胆固醇水平无明显区别。与空白组、手术组、参芎葡萄糖组相比,辛伐他汀组血清胆固醇水平降低(P<0.05)。与空白组、手术组相比,参芎葡萄糖组血清胆固醇水平降低(P>0.05)。参芎葡萄糖+辛伐他汀组血清胆固醇水平低于辛伐他汀组,但无统计学差异(P>0.05)。HE染色结果表明手术组血管内膜较空白组增厚(P<0.05),参芎葡萄糖组血管内膜增生较手术组及辛伐他汀组减少(P<0.05),参芎葡萄糖+辛伐他汀组血管内膜增生较参芎葡萄糖组减少,但无统计学差异(P>0.05)。免疫组织化学结果表明参芎葡萄糖组内膜中膜PCNA、MMP9阳性细胞率较手术组均降低(P<0.05),参芎葡萄糖组MMP9、PCNA阳性细胞率较辛伐他汀组均降低,但无统计学差异(P>0.05),参芎葡萄糖+辛伐他汀组MMP9、PCNA阳性细胞率较参芎葡萄糖组降低,但无统计学差异(P>0.05)。Western blot结果表明参芎葡萄糖组GRP78表达量较手术组、辛伐他汀组均降低(P<0.05)。结论参芎葡萄糖注射液可有效减缓球囊损伤后血管内膜增生。
Objective To observe the effect of shenxiong glucose injection on intimal hyperplasia of rabbitvascular after carotid artery balloon injury. Methods 35 four months old healthy male New Zealand rabbits wererandomly divided into control group, operation group, simvastatin group, shenxiong glucose group, shenxiongglucose＋ simvastatin group. Each group had 7 rabbits. Two weeks after operation and drug intervention, serumcholesterol level were measured. Hematoxylin and eosin staining （HE） was used to observe intimal hyperplasia;immunohistochemical staining was used to analyze intimal proliferation cell nuclear antigen （PCNA） and matrixmetalloproteinase-9 （MMP-9） expression, Western blot was used to analyze carotid glucose-regulated protein78 （GRP78） expression. Results There was no different in serum cholesterol levels between operation group andcontrol group. Serum cholesterol levels of simvastatin group was lower than control group, operation group andshenxiong glucose group （P〈0.05）. Serum cholesterol levels of shenxiong glucose group was lower than controlgroup and operation group （P〈0.05）. Serum cholesterol levels of shenxiong glucose＋ simvastatin group was lowerthan simvastatin group with no significant difference （P〉0.05）. HE result showed that intima of operation groupwas thicker than control group （P〈0.05）; intima hyperplasia of shenxiong glucose group was reduced than operationgroup and simvastatin group （P〉0.05）; intima hyperplasia of shenxiong glucose＋ simvastatin group was reduced thanshenxiong glucose group with no significant difference （P〉0.05）. Immunohistochemical analysis results showed thatPCNA, MMP9 positive cells in shenxiong glucose group was reduced than operation group （P〈0.05）; PCNA, MMP9positive cells in shenxiong glucose group was reduced than simvastatin group with no significant difference （P〉0.05）;PCNA, MMP9 positive cells in shenxiong glucose＋ simvastatin group was reduced than shenxiong glucose groupwith no significant difference （P〉0.05）. GRP78 expression in shenxiong glucose group was reduced than operationgroup and simvastatin group according to result of Western blot （P〈0.05）. Conclusion The Shenxiong glucose caneffectively reduce neointimal hyperplasia after balloon injury.
出处
《中国循证心血管医学杂志》
2016年第5期619-623,共5页
Chinese Journal of Evidence-Based Cardiovascular Medicine
关键词
参芎葡萄糖注射液
球囊损伤
血管内膜增生
Shenxiong glucose injection
Balloon endothelial injury
Intimal hyperplasia
