摘要
目的建立湿法回流消解-氢化物-原子荧光光谱法测定营养强化剂中总硒的含量。方法采用硝酸-高氯酸（4：1,V：V）作为消解体系230℃回流消解,采用氢化物-原子荧光光谱法进行测定;并考查消解体系、还原剂和掩蔽剂等对荧光强度的影响。结果采用4.0%盐酸作为酸介质,硼氢化钾浓度为2.0%时荧光强度最强。硒在浓度范围为2.0~40.0 ng/mL呈现良好的线性关系,相关系数为0.9999,方法检出限为0.028 ng/mL,加标回收率为93.0%~102.0%,相对标准偏差为2.2%（n=6）。结论该方法试剂用量少、消解速度快、准确度好、灵敏度高,适合于富硒营养强化剂中总硒含量的测定。
Objective To establish a method for the determination of total selenium in nutrition enhancer by hydride generation-atomic fluorescence spectrometry（HG-AFS） with refluxing wet digestion.Methods The nitric acid-perchloric acid（4：1,V：V） was used as the digestion system with reflux digestion temperature of 230 ℃.Samples were detected by the method of hydride generation-atomic fluorescence spectrometry.And then the effects of digestion system,reducing agent and masking agent on fluorescence intensity were investigated.Results The fluorescence intensity was the strongest using 4.0%hydrochloric acid as acid medium and 2.0%potassium borohydride.Selenium had a good linear relationship in the range of 2.0~40.0 ng/mL with the correlation coefficient of 0.9999.The limit of detection was 0.028 ng/mL.The recovery rates at 3 spiked levels were in the range of93.0%~102.0%,and RSD was 2.2%（n=6）.Conclusion This method is simple,rapid,and accurate,and reliable for the determination of total selenium in selenium nutrition enhancer.
出处
《食品安全质量检测学报》
CAS
2016年第5期1808-1812,共5页
Journal of Food Safety and Quality
关键词
富硒营养强化剂
总硒
氢化物-原子荧光光谱法
selenium nutrition enhancer
total selenium
hydride generation-atomic fluorescence spectrometry（HG-AFS）
