摘要
目的建立高效液相色谱（HPLC）测定口服液中左旋肉碱含量的方法。方法样品经0.5 mmol/L盐酸溶液超声提取,采用CAPCELL PAK ACR C_（18）柱（250 mm×4.6 mm,5μm）,以0.002 mol/L辛烷磺酸钠溶液（加入0.125%磷酸二氢钾、0.125%磷酸氢二钾,用磷酸调pH3.0）-7腈（92：8,V：V）为流动相,流速1.0 mL/min,检测波长：210 nm,柱温：25℃。结果左旋肉碱的浓度在150.1~1800.7μg/mL范围内呈良好的线性关系（r=0.9999）,平均加样回收率为95.50%,RSD为2.0%（n=6）。结论本方法快速、准确、重复性好,可作为口服液类辅助降血脂保健食品的质量控制方法。
Objective To establish a method for the determination of L-carnitine in health foods by high performance liquid chromatography（HPLC）.Methods The separation was performed on a CAPCELL PAK ACR C_（18） column with the mixture of acetonitrile-0.002 mol/L 1-octanesulfonic acid solution（8：92,V：V） containing 0.125%potassium dihydrogen phosphate and 0.125%dipotassium phosphate with pH 3.0 as mobile phase.Detection wavelength was 210 nm and flow rate was 1.0 mL/min with the column temperature of 25 ℃.Results The linear range for L-carnitine was from 150.1 μg/mL to 1800.7 μg/mL,and the average recovery was 95.50%） with a RSD of2.0%）（n=6）.Conclusions This method is accurate,simple and sensitive,and can be used for the quality control of the health foods with oral liquids.
出处
《食品安全质量检测学报》
CAS
2016年第5期2093-2096,共4页
Journal of Food Safety and Quality
关键词
高效液相色谱
保健食品
左旋肉碱
high performance liquid chromatography
health food
L-carnitine
