摘要
利用20个微卫星位点,采用荧光-多重PCR分型技术,对4个野生东方田鼠群体(福建、湖南、宁夏、广西)进行遗传结构和遗传分化检测。结果表明:19个微卫星位点显示出高度多态性(PIC=0.83>0.5),平均等位基因数、位点杂合度均显示4个地区野生东方田鼠的遗传多样性丰富。F统计量表明:4个野生东方田鼠群体的遗传差异有13.5%来自于群体间,86.5%的遗传差异主要来源于个体间。遗传距离与UPGMA聚类分析均表明,宁夏群体与广西群体间的遗传分化程度最大,湖南群体与广西群体间的遗传分化程度最小,这在STRUCTURE聚类图分析时(K=2)同样得到提示。分析认为,广西群体与湖南群体间的遗传分化较小,存在一定基因交流的原因是这两个群体间较近的地理距离。
Based on 20 microsatellite loci,the genetic structure and genetic differentiation of 4 wild Microtus fortis populations（Fujian,Hunan,Guangxi and Ningxia）were detected by multiplex fluorescence PCR technique.The results indicated that 19 microsatellite loci showed high polymorphism（PIC=0.830.5）,and the average number of alleles and loci heterozygosity showed that the genetic diversity was abundant in 4 regions of M.fortis.F-statistic showed that the genetic difference among the populations was 13.5%,and 86.5%of genetic difference was mainly from individuals.Genetic distances and UPGMA cluster analysis showed that the degree of genetic differentiation between Ningxia population and Guangxi population was the largest,the degree of genetic differentiation between Hunan population and Guangxi population was the least,which was also prompted in STRUCTURE clustering graph analysis（K=2）.The genetic differentiation between Guangxi population and Hunan population was small,the reason for the existence of a certain gene exchange is the closer geographical distance between the two populations.
出处
《上海农业学报》
CSCD
2016年第3期72-77,共6页
Acta Agriculturae Shanghai
基金
上海市科委项目(09140900101
09140900102)
关键词
东方田鼠
微卫星位点
遗传多样性
Microtus fortis
Microsatellite loci
Genetic diversity
