摘要
目的:研究重复经颅磁刺激(rTMS)对脑梗死大鼠空间记忆功能的影响,并探讨其可能机制。方法:45只大鼠随机分为假手术组、对照组和rTMS组各15只,采用大脑中动脉栓塞再灌注(tMCAO)法制作脑梗死大鼠模型。采用Morris水迷宫评定学习记忆功能变化,通过实时定量聚合酶链式反应(qRT-PCR)观察患侧海马白细胞白介素1β(IL-1β)、诱导型一氧化氮合酶(iNOS)、肿瘤坏死因子(TNF-α)以及梗死灶区(IL-1β)mRNA表达量。结果:与假手术组相比,对照组及rTMS组大鼠逃避潜伏期显著延长(P<0.01),而rTMS组较对照组的逃避潜伏期则明显缩短(P<0.05)。对照组、rTMS组IL-1βmRNA在患侧海马及皮层的表达量均较假手术组增高(P<0.01),rTMS组患侧海马IL-1βmRNA表达较对照组显著增高(P<0.01),患侧皮层IL-1βmRNA表达较对照组无明显变化。对照组、rTMS组iNOS、TNF-αmRNA表达量均较假手术组增高(P<0.01),rTMS组与对照组无明显差异。结论:rTMS治疗可促进脑梗死后空间学习记忆功能恢复,并可能通过增加患侧海马区IL-1βmRNA表达来实现。
Objective：To investigate the effects of rTMS in high frequency on the recovery of spatial memory function and the expression of interleukin-1beta（IL-1β）mRNA in ipsilesional hippocampus of rats subjected to focal cerebral ischemia.Methods：A rat model of acute focal cerebral ischemia was established using transient middle cerebral artery occlusion technique.Forty-five rats were randomly assigned to sham operative group,ischemic control group and rTMS group.Spatial memory function was evaluated by Morris water maze.The expression of pro-inflammatory cytokines（IL-1β,iNOS,TNF-α）in ipsilesional hippocampus and ischemic core was detected by real-time quantitative polymerase chain reaction.Results：The escape latency in ischemic control group and rTMS group was delayed more than that in sham operative group（P〈0.01）.The escape latency in rTMS group was significantly shortened as compared with that in model control group（P〈0.05）.The expression of IL-1β,iNOS and TNFαmRNA was increased in ischemic group and rTMS group（P〈0.01）as compared with sham operative group（P〈0.01）.In the hippocampus,the expression of IL-1βmRNA in rTMS group was significantly increased as compared with that in the ischemic control group（P〈0.01）.In the ipsilateral cortex,there was no significant difference in the expression of IL-1βmRNA between rTMS group and ischemic control group.Conclusion：rTMS can promote the recovery of spatial memory function,which was possibly achieved by increasing the expression of IL-1βmRNA in the ipsilateral hippocampus.
出处
《中国康复》
2016年第3期165-167,共3页
Chinese Journal of Rehabilitation
基金
广东省自然科学基金博士启动项目(S2013040013489)
关键词
经颅磁刺激
脑缺血
空间记忆
IL-1β
transcranial magnetic stimulation
cerebral ischemia
spatial memory
interleukin-1
