摘要
微卫星分子标记技术被广泛应用于分子生物学研究中,具有多态性高、重复性好、共显性表达、杂合度高等特点,在种群遗传多样性、遗传图谱构建等领域发挥不可替代的作用。近年来,随着新一代高通量测序技术的进一步成熟,简单重复序列(simple sequence repeat,SSR)标记的开发技术与应用得到了进一步的发展。现主要对基于高通量测序的微卫星分子标记最新开发技术进行介绍,并从遗传多样性分析、遗传图谱构建、品种鉴定及分子辅助育种等方面,总结近年来SSR标记技术在经济植物研究中的最新应用,最后对SSR技术的应用前景进行展望,以期为利用微卫星技术进行经济植物研究提供参考。
Microsatellite or simple sequence repeat （SSR）, as a molecular marker technology, has been widely applied in molecular biology researches. With its advantages of high polymorphism, repeatability, codominance, and high heterozygosity, SSRs plays important roles in studies of population genetic diversity and genetic mapping. Recently, with rapidly developing high-throughput sequencing, the method of development and application of SSR marker technology has been improved. The new technology of SSR development based on high-throughput sequencing was introduced here, and SSR applications in genetic diversity, ge- netic mapping, cuhivar identification, and molecular breeding in recent studies of economic plants were re- viewed. It aims to give a view of the application of microsatellite markers and provide references for economic plant research using this technology.
出处
《生命科学研究》
CAS
CSCD
2016年第3期260-266,共7页
Life Science Research
基金
国家自然科学基金资助项目(31400194)
科技部基础性工作专项(2015FY110200)
浙江理工大学科研启动基金(14042010-Y)
浙江理工大学校科创基金
浙江省人保厅2014留学人员科技活动项目
关键词
简单序列重复
高通量测序
微卫星标记开发
植物遗传多样性
表达序列标签
转录组
simple sequence repeat （SSR）
high-throughput sequencing
microsatellite marker development
plant genetic diversity
expressed sequence tags （EST）
transcriptome
