摘要
为了探讨牛磺鹅去氧胆酸(TCDCA)对小鼠单核巨噬细胞系RAW264.7的抗凋亡作用,试验采用二苯胺法及实时荧光定量PCR技术分别检测了针对RAW264.7细胞凋亡百分率及凋亡抑制蛋白CIAP-1、CIAP-2和XIAP的mRNA表达影响。结果显示,剂量为0.05、0.10和10μg/mL TCDCA可以极显著地对抗地塞米松(DEX)诱导的RAW264.7细胞系凋亡(P<0.01)。1μg/mL TCDCA对正常RAW264.7细胞系CIAP-1和XIAP表达有显著的促进作用(P<0.05);10μg/mL TCDCA对正常RAW264.7细胞系CIAP-1、CIAP-2和XIAP表达均具有显著的促进作用(P<0.05)。TCDCA给药后对DEX诱导的RAW264.7细胞系CIAP-1、CIAP-2和XIAP表达均具有极显著的促进作用(P<0.01),但不同给药剂量的TCDCA作用有所差异。以上研究结果表明,TCDCA具有对抗DEX诱导的小鼠巨噬细胞系RAW264.7凋亡作用,且与上调凋亡抑制蛋白mRNA表达有关。
We carried out the research of the effect of taurochenodeoxycholic acid （TCDCA）on the apoptosis of the mouse macrophage cell line RAW264.7 and expression of IAPs including CIAP-1,CIAP-2 and XIAP.The results showed that TCDCA with the dose of 0.05,0.10 and 10μg/mL could extremely significantly prevent cells apoptosis that were induced by DEX （P〈0.01）.TCDCA with the dose of 1μg/mL could significantly promote mRNA expressions of CIAP-1 and XIAP （P〈0.05）,while the dose of 10μg/mL could significantly promote mRNA ex-pressions of CIAP-1,CIAP-2 and XIAP （P〈0.05）,TCDCA could also extremely significantly up-regulate the expression of CIAP-1,CIAP-2 and XIAP （P〈0.01）in RAW264.7 cell line by DEX, but this depended on different doses of TCDCA.The above research results showed that TCDCA could resist the cells apoptosis of the mouse macrophage cell line RAW264.7 that were induced by DEX,which was related with the influence on the mRNA expression of IAPs.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第7期1674-1680,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(30800837)
山东省高等学校科技计划项目(J13LE53)
山东省博士后基金(76404)
