摘要
目的:探讨CD44v17对宫颈癌的临床诊断意义。方法:将CD44v17si RNA、CD44v17、生理盐水转染至传代后的人宫颈癌细胞。检测细胞转染后存活率;检测细胞凋亡率。在裸鼠左肩背部注入人宫颈癌细胞悬液,随机分为CD44v17组、CD44v17si RNA组、对照组。在CD44v17组、CD44v17si RNA组裸鼠瘤体内分别注入CD44v17病毒颗粒、CD44v17si RNA病毒颗粒。检测瘤体的质量与体积。选取疑有宫颈病变患者阴道镜下活检组织80例,正常宫颈组织15例、宫颈上皮内瘤变(CIN)I级组织l5例、CIN II级15例、CIN III级组织15例和宫颈癌组织20例。检测CD44v17在不同组织中的表达量。结果:CD44v17si RNA转染的宫颈癌细胞凋亡率(19.20±2.14%)高于CD44v17转染的宫颈癌细胞凋亡率(6.13±1.08%)(P<0.05)。CD44v17组裸鼠瘤体质量(15.9±3.4)g高于对照组裸鼠瘤体质量(11.8±2.7)g(P<0.05)。CD44v17在不同组织中的表达量,按正常宫颈、CINⅠ级、CINⅡ级、CINⅢ级、宫颈癌发展过程呈递增趋势(P<0.05)。结论:CD44v17能抑制宫颈癌细胞凋亡,促进宫颈癌细胞的生长、增殖。通过降低CD44v17表达量可能是遏制CIN向宫颈癌发展的一个手段。
Objective： To explore the clinical diagnosis value of CD44V17 in cervical cancer. Methods： CD44v17 si RNA, CD44v17,physiological saline was transfected into cultured human cervical cancer cells. The cell viability was detected. The rate of apoptosis was detected. Human cervical cancer cell was suspensioned the left shoulder of nude mice. The nude mice were randomly divided into CD44v17 group, CD44v17 si RNA group and control group. The nude mice of CD44v17 group and CD44v17 si RNA group were injected with CD44v17 virus particles and CD44v17 si RNA virus particles, respectively. The quality and volume of tumor were detected. The biopsy tissues of totally 80 patients with cervical lesions were selected. According to pathological classification, there were 15 cases of normal cervical tissues, cervical intraepithelial neoplasia（CIN） I grade 15 cases, II grade CIN 15 cases, III grade CIN 15 cases and 20 cases of cervical cancer tissues. The expression of CD44v17 in different tissues was detected. Results： The rate of apoptosis of cervical cancer cells transfected with CD44v17 si RNA（19.20±2.14 %） was higher than that of CD44v17 transfected cervical cancer cell（6.13±1.08 %）（P 〈0.05）. The quality of tumor（15.9±3.4） g in CD44v17 group was higher than that in control group（11.8 ±2.7） g（P〈0.05）.The expression of CD44v17 in normal cervix, cervical intraepithelial neoplasia grade, grade II CIN, cervical intraepithelial neoplasia grade, cervical cancer was progressively increased with statistical significance（P〈0.05）. Conclusions： CD44v17 can inhibit the apoptosis of cervical cancer cells and promote the growth and proliferation of cervical cancer cells. By reducing the expression of CD44v17 may be a effective method to curb the development of CIN to cervical cancer.
出处
《现代生物医学进展》
CAS
2016年第19期3625-3628,3609,共5页
Progress in Modern Biomedicine
基金
上海市卫生局科研基金项目(03.02.12.003)
